Purified anti-PADI4 Antibody

Pricing & Availability
Clone
O94H5 (See other available formats)
Regulatory Status
RUO
Other Names
Peptidylarginine deiminase 4, Peptidyl Arginine Deiminase, Type IV, PADI5, HL-60 PAD, Protein-Arginine Deiminase Type-4, PAD4
Isotype
Mouse IgG2a
Ave. Rating
Submit a Review
Product Citations
publications
O94H5_PURE_PADI4_Antibody_1_031919
Whole cell extracts (15 µg protein) from HL-60 cells treated (+) or untreated (-) with 1.25% DMSO for 5 days were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL of Purified anti-PADI4 Antibody (1:500 dilution) Antibody, clone O94H5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
  • O94H5_PURE_PADI4_Antibody_1_031919
    Whole cell extracts (15 µg protein) from HL-60 cells treated (+) or untreated (-) with 1.25% DMSO for 5 days were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with 1.0 µg/mL of Purified anti-PADI4 Antibody (1:500 dilution) Antibody, clone O94H5, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
684202 100 µg $245
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

PADI4 is an enzyme that catalyzes peptidyl arginine to citrulline in the presence of Ca2+ ions. This reaction, known as citrullination, leads to the post-translational modification of proteins. PADI4 was initially cloned from the HL-60 cell line (human promyelocytic leukemia cells). It is mainly expressed in granulocytes, monocytes and CD34+ stem cells. PADI4 may play a role in granulocyte and macrophage development leading to inflammation and immune response. The PADI4 single-nucleotide polymorphism (SNP) and haplotypes were associated with rheumatoid arthritis susceptibility. Recently, PADI4 has also been found in a variety of malignant tumors and implies its potential roles in tumorigenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein of human PADI4 expressed in HEK293T.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kang L, et al. 2020. Nat Commun. 11:2488. PubMed
  2. Vaibhav K, et al. 2020. Sci Adv. 6:eaax8847. PubMed
RRID
AB_2572090 (BioLegend Cat. No. 684202)

Antigen Details

Structure
663 amino acid with the predicted molecular weight of approximately 74kD.
Distribution

PADI4 is mainly expressed in granulocytes, monocytes and CD34+ stem cells.

Function
PADI4 may play a role in granulocyte and macrophage development leading to inflammation and immune response.
Interaction
Histone H1, Histone H3, Histone H4, and SOX4.
Cell Type
Granulocytes, Hematopoietic stem and progenitors, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity
Antigen References

1. Abd-Allah SH, et al. 2012. Joint Bone Spine 79:124.
2. Worthington J, et al. 2003. Trends Mol. Med. 9:405.
3. Song G, et al. 2016. Oncotarget. 7:3144-57.
4. Kolodziej S, et al. 2014. Nat. Commun. 5:3995.
5. Suzuki A, et al. 2003. Nat. Genet. 34:395.
6. Chang X, et al. 2010. Cancer Cell International 10:7.

Gene ID
23569 View all products for this Gene ID
UniProt
View information about PADI4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account