Purified anti-O-GlcNAc Antibody

Pricing & Availability
Clone
RL2 (See other available formats)
Isotype
Mouse IgG1, λ
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Product Citations
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RL2_PURE_O-GlcNAc_Antibody_WB_062215
Same amount (15 µg) of total protein harvested from HeLa, NIH3T3, rat E18 brain, and mouse brain were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal O-GlcNAc (clone RL2) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • RL2_PURE_O-GlcNAc_Antibody_WB_062215
    Same amount (15 µg) of total protein harvested from HeLa, NIH3T3, rat E18 brain, and mouse brain were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal O-GlcNAc (clone RL2) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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677902 100 µg $295
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Description

The monoclonal antibody RL2 reacts specifically with beta-O-linked N-acetylglucosamine (O-GlcNAc). It recognizes o-GlcNAc in beta O-glycosidic linkage to both serine and threonine. However, it exhibits no cross reactivity with alpha-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin. This antibody readily detects in vivo changes in O-GlcNAc modification1.

O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all metazoans2. O-GlcNAcylation is similar to protein phosphorylation. Research on O-GlcNAc is particularly relevant to chronic human diseases such as diabetes, cardiovascualr disease, neurodegenerative disorders, and cancer3.

Like phosphorylation, the specific functions of O-GlcNAc depend on the protein and sites to which the glycan is attached2. One of the major functions of O-GlcNAc is to prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress4.

Product Details
Technical data sheet

Product Details

Reactivity
Beta-O-linked N-acetylglucosamine (O-GlcNAc) in all metazoans including plants, animals, and the viruses that infect them.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
O-GlcNAc in beta-O-glycosidic linkage to both serine and threonine.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IF, IHC, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Snow CM, et al. 1987. J. Cell Biol. 104:1143.
  2. Shin SH, et al. 2011. Amino Acids 40:885-93.
  3. Mi W, et al. 2011. Biochim. Biophys. Acta. 4:514.
  4. Hoedt E, et al. 2010. J. Biol. Chem. 285:19205.
RRID
AB_2565912 (BioLegend Cat. No. 677902)

Antigen Details

Structure
O-GlcNAc modified protein.
Distribution
Nucleus and cytoplasm.
Function
To prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress.
Biology Area
Cell Biology, Signal Transduction, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Comer FI, et al. 2001. Anal Biochem. 293:169.
2. Hart, GW and Akimoto, Y. (2009) Essentials of Glycobiology, 2nd edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
3. de Queiroz RM, et al. 2014. Front Oncol. 4:132.
4. Hart GW, et al. 2014. Cell Metab. 20:208.

Gene ID
NA
UniProt
View information about O-GlcNAc on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/23/2015

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