- Clone
- N97A/31 (See other available formats)
- Other Names
- Neuroligin-1, neuroligin I
- Previously
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Covance Catalog# MMS-5165
- Isotype
- Mouse IgG1
- Ave. Rating
- 0 reviews

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IHC staining of purified anti-Neuroligin-1 antibody (clone N97A/31) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Neuroligin-1 antibody (clone N97A/31) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Neuroligin-1 antibody (clone N97A/31) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Neuroligin-1 antibody (clone N97A/31) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Avail. | Save | ||
---|---|---|---|---|---|---|
819001 | 100 µl | $140.00 |
Neuroligin is a postsynaptic membrane protein that binds to Neurexin, helping to form and maintain neuronal synapses. Within the postsynaptic neuron, it binds to proteins such as PSD-95, which recruits receptors and channels to the synapse.
Product DetailsProduct Details
- Reactivity
- Human, Mouse, Rat
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 718-843 of rat Neuroligin-1 protein.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody is effective in formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).
- RRID
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AB_2564826 (BioLegend Cat. No. 819001)
Antigen Details
- Structure
- Expected MW: 120 kD
- Gene ID
- 116647 View all products for this Gene ID
- UniProt
- View information about Neuroligin-1 on UniProt.org
Related Pages & Pathways
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Related FAQs
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