Purified anti-Neurofilament L (NF-L) Antibody

Pricing & Availability
Clone
NFL2 (See other available formats)
Other Names
Neurofilament Light Polypeptide 68kD, Neurofilament Light Polypeptide, Neurofilament Subunit NF-L, CMT1F, NFL, NEFL, NF-L
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NFL2_Pure_NeurofilamentL_Antibody_0_092619
Western blot of purified anti-Neurofilament L (NF-L) antibody (clone NFL2). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NFL2_Pure_NeurofilamentL_Antibody_0_092619
    Western blot of purified anti-Neurofilament L (NF-L) antibody (clone NFL2). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NFL2_Pure_NeurofilamentL_Antibody_1_092619
    Western blot of purified anti-Neurofilament L (NF-L) antibody (clone NFL2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cerebellum lysate; Lane 3: 20 µg of human brain cortex lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NFL2_Pure_NeurofilamentL_Antibody_2_IHC_041719
    IHC staining of purified anti-Neurofilament L (NF-L) antibody (clone NFL2) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
846002 100 µg $225
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Description

Neurofilaments (NF) are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Detection of NF, and the subunits of NF, may serve as a biomarker for axonal degeneration. The degree of axonal degeneration is related to the amount of NF detected in the cerebrospinal fluid (CSF) and the blood. Neurofilament light (NF-L) levels are elevated in Alzheimer’s Disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified macaque NFL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg/ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Park YG, et al. 2018. Nat Biotechnol. 37:73. PubMed
Publication Library
RRID
AB_2566775 (BioLegend Cat. No. 846002)

Antigen Details

Structure
Neurofilament light is a 68 kD protein.
Distribution

Cytoskeleton and the cytoplasm.

Function
Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Zetterberg H, Blennow K. 2015. JAMA Neurol. 11:1237-8.
2. Bäckström D, et al. 2015. JAMA Neurol. 72(10):1175.
3. Janelidze S, et al. 2015. Neurology 85:1834.
4. Magdalinou NK, et al. 2015. J. Neurol. Neurosurg. Psychiatry 86(11):1240.
5. Skillbäck T, et al. 2014. Neurology. 83:1945.

Gene ID
4747 View all products for this Gene ID
UniProt
View information about Neurofilament L (NF-L) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/26/2019

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