Purified anti-mouse Ly-49C/F/I/H Antibody

Pricing & Availability
Clone
14B11 (See other available formats)
Other Names
Ly-49 inhibitory receptors C, F and I and the activating receptor H
Isotype
Syrian Hamster IgG
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Product Citations
publications
14B11_Purified_091207
C57BL/6 mouse splenocytes stained with purified 14B11, followed by anti-Armenian hamster IgG FITC
  • 14B11_Purified_091207
    C57BL/6 mouse splenocytes stained with purified 14B11, followed by anti-Armenian hamster IgG FITC
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108202 500 µg $200
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Description

The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also known as Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with other Ly-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H is expressed on a subset of NK cells, NK-T cells and some CD8+ cells. Ly-49C/F/I, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain an ITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1r gene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c mice are reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NK cells, is capable of inducing redirected cell lysis and blocking NK cell effector function.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
IL-2-activated killer cells (LAK) from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes

Additional reported applications (for the relevant formats) include: induction of redirected lysis1, and blocking2 of the binding of H-2d lymphoblasts to transfectants expressing Ly-49C, Ly-49F, and Ly-49I.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested for the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California. 

Application References

(PubMed link indicates BioLegend citation)
  1. Corral L, et al. 1999. Hybridoma 18:359.
  2. Hanke T, et al. 1999. Immunity 11:67.
RRID
AB_313351 (BioLegend Cat. No. 108202)

Antigen Details

Structure
Ly-49 family, 110 kD
Distribution
NK and NK-T cells, some CD8+ T cells
Function
NK cell activation or inhibition
Ligand Receptor
MHC Class I Molecules
Cell Type
NK cells, NKT cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Ryan JC, et al. 1997. Immunol. Rev. 155:79.
2. Mason LH, et al. 1997. J. Immunol. 159:4187.
3. Lanier LL. 1998. Ann. Rev. Immunol. 16:359.
4. Yokoyama W, et al. 1993. Ann. Rev. Immunol. 11:613.

Gene ID
16634 View all products for this Gene ID
UniProt
View information about Ly-49C/F/I/H on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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