- 5H10-1 (See other available formats)
- Other Names
- T8, Lyt2, Ly-2
- Rat (SD) IgG2b, λ
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- Product Citations
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CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.Product Details
- Antibody Type
- Host Species
- Concanavalin A-stimulated BALB/c splenic T cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
ICC - Validated
IHC-F - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, immunohistochemistry of acetone-fixed frozen sections2, and immunofluorescence. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.
Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.
(PubMed link indicates BioLegend citation)
- Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557.
- Frei K, et al. 1997. J. Exp. Med. 185:2177.
- Product Citations
AB_312762 (BioLegend Cat. No. 100801)
AB_312763 (BioLegend Cat. No. 100802)
- Ig superfamily, CD8α chain, 34 kD
Most thymocytes, T cell subset, some NK cells
- Co-receptor for TCR
- MHC class I molecule
- Cell Type
- Dendritic cells, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
- Gene ID
- 12525 View all products for this Gene ID
- View information about CD8a on UniProt.org
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