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Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
W21115A (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W21115A_PURE_CD326_IHC-F_092423
IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on frozen mouse uterus section. C57BL/6 mouse frozen uterus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
  • a. W21115A_PURE_CD326_IHC-F_092423
    IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on frozen mouse uterus section. C57BL/6 mouse frozen uterus section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
  • b. W21115A_PURE_CD326_IHCP_092523
    IHC staining of purified anti-mouse CD326 (Ep-CAM) (clone W21115A) on formalin-fixed paraffin-embedded C57BL/6 mouse intestine tissue. Following antigen retrieval using 1X citrate buffer 1X (10 mM, pH 6.0) (Cat. No. 420901), the tissue was incubated without (panel A) or with (panel B) 1 µg/mL of purified anti-mouse CD326 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
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114202 100 µg $375
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein aa24-266
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemical staining on frozen tissue sections, a concentration range of 2.5 - 10.0 µg/mL is suggested. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types

Function
Mediates calcium-independent homophilic cell-cell adhesion
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Borkowski TA, et al. 1996. Eur J Immunol. 26:110-4.
  2. Bergsagel PL, et al. 1992. J Immunol. 148:590-6.
Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/18/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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