Purified anti-MMP-9 Antibody (Previously Covance catalog# MMS-5163)

Pricing & Availability
Clone
L51/82 (See other available formats)
Other Names
GELB, MMP9, gelatinase B, 92 kD gelatinase, 92 kD type IV collagenase, 92-kD type IV collagenase, matrix metalloproteinase 9 (gelatinase B 92-kD type IV collagenase), matrix metalloproteinase 9 (gelatinase B, 92-kD type IV collagenase)
Previously
Covance Catalog# MMS-5163
Isotype
Mouse IgG2a
Ave. Rating
0 reviews
Product Citations
publications
L51slash82_Purified_MMP-9_Antibody_1_040618
IHC staining of purified anti-MMP9 antibody (clone L51/82) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • L51slash82_Purified_MMP-9_Antibody_1_040618
    IHC staining of purified anti-MMP9 antibody (clone L51/82) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • L51slash82_Purified_MMP-9_Antibody_2_040618
    IHC staining of purified anti-MMP9 antibody (clone L51/82) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L51slash82_Purified_MMP-9_Antibody_3_040618
    Western blot of purified anti-MMP9 antibody (clone L51/82). Lanes 1: Molecular weight marker; Lanes 2:20 µg of human spleen lysate; Lanes 3:20 µg of rat spleen lysate. The blots were incubated with 5 µg/mL of clone L51/82 or mouse IgG2a overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • L51slash82_Purified_MMP-9_Antibody_4_040618
    Western blot of purified anti-MMP9 antibody (clone L51/82). Lanes 1: Molecular weight marker; Lanes 2:20 µg of mouse spleen lysate. The blots were incubated with 1 µg/mL of clone L51/82 or mouse IgG2a overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
819701 100 µl $140.00
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Description

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes different groups of enzymes, such as, collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades proteins in the extracellular matrix and activates growth factors like proTGFb and proTNFa. MMP-9 contributes to the invasion and metastasis of various human malignancies. Clone F11P2C3 has been shown to be useful for western blotting and immunohistochemistry of human MMP9.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a full-length fusion protein corresponding to amino acids 1-708 of rat MMP9 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

RRID
AB_2564833 (BioLegend Cat. No. 819701)

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Go To Top Version: 3    Revision Date: 04/06/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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