Purified anti-IP3R1 Antibody (Previously Covance catalog# MMS-5151)

Pricing & Availability
Clone
L24/18 (See other available formats)
Other Names
Inositol 1,4,5-trisphosphate receptor type 1, IP-3-R, IP3R 1, IP3 receptor, type 1 InsP3 receptor, inositol 1,4,5-triphosphate receptor 1, inositol 1,4,5-triphosphate receptor, type 1, type 1 inositol 1,4,5-trisphosphate receptor
Previously
Covance Catalog# MMS-5151
Isotype
Mouse IgG1
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Product Citations
publications
1_L24slash18_Purified_IP3R1_Antibody_IHC_030415
IHC staining of anti-IP3R1 antibody (clone L24/18) on Formalin Fixed Paraffin Embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_L24slash18_Purified_IP3R1_Antibody_IHC_030415
    IHC staining of anti-IP3R1 antibody (clone L24/18) on Formalin Fixed Paraffin Embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_L24slash18_PURE_IP3R1_Antibody_WB_121917
    Western blot of anti-IP3R1 antibody (clone L24/18). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • 3_L24slash18_Purified_IP3R1_Antibody_IHC_2_121917
    IHC staining of anti-IP3R1 antibody (clone L24/18) on Formalin Fixed Paraffin Embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
  • 4_L24slash18_Purified_IP3R1_Antibody_IHC_3_121917
    IHC staining of anti-IP3R1 antibody (clone L24/18) on Formalin Fixed Paraffin Embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm.
Cat # Size Price Quantity Avail. Save
817701 100 µl $140.00
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Description

Inositol 1,4,5-triphosphate (IP3) is a calcium-regulating second messenger that receptor involved in ER stress-induced apoptosis. IP3 receptor, type 1 (IP3R1) is a receptor for IP3 expressed on the ER which opens a calcium channel on the ER after IP3 bidning. IP3R1 is expressed in developing and mature neurons and its dysregulation may be involved in progression of certain neurodegenerative diseases. IP3R1 is also involved in autophagy, cellular energenetics, macrophage inflammasomes, and other cellular processes.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 2680-2749 of rat IP3 receptor type 1.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P, WB

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition. 
• WB: 1-10 µg/ml
• IHC: 1-10 µg/ml

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

This antibody is less reactive in human samples than mouse and rat sample in WB.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhao G, et al. 2010. J. Gen. Physiol. 136(3):283-91 (WB)
Product Citations
  1. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
Publication Library
RRID
AB_2564813 (BioLegend Cat. No. 817701)

Antigen Details

Structure
IP3R1 is a 2758 amino acid protein with an apparent molecular mass of 314 kD.
Distribution

Cellular distribution: Plasma membrane, endoplasmic reticulum, and nucleus.

Antigen References

Tang T-S, et al. 2009. J. Neurosci. 29 (5) 1257-66.

Gene ID
25262 View all products for this Gene ID
UniProt
View information about IP3R1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/10/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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