Purified anti-human CD22 Antibody

Pricing & Availability
Clone
A21052D (See other available formats)
Regulatory Status
RUO
Other Names
BL-CAM, Siglec-2, Lyb8
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A21052D_PURE_CD22_Antibody_033022
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD22 (clone A21052D) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • A21052D_PURE_CD22_Antibody_033022
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD22 (clone A21052D) and purified anti-human IgD (clone W18340A) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse IgG (clone Poly4053) (red) and Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054) (green) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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382701 25 µg $55
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382702 100 µg $121
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Description

CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM and is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested.  It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927812 (BioLegend Cat. No. 382701)
AB_2927812 (BioLegend Cat. No. 382702)

Antigen Details

Structure
Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
Distribution

B cells

Function
Adhesion, B-monocyte and B-T interactions
Ligand/Receptor
CD45RO, CD75
Biology Area
Cell Adhesion
Molecular Family
Adhesion Molecules
Antigen References
  1. Clark E. 1993. J Immunol. 150:4715-8.
  2. Shan D, et al. 1995. J Immunol. 154:4466-75.
Gene ID
933 View all products for this Gene ID
UniProt
View information about CD22 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/30/2022

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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  • Purified anti-human CD22

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