Purified anti-human CCL17 (TARC) Antibody

Pricing & Availability
Clone
Poly5230 (See other available formats)
Other Names
Thymus and activation-regulated chemokine (TARC), Small-inducible cytokine 17 (SCYA17), ABCD-2
Isotype
Goat Polyclonal Ig
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Poly5230_PURE_CCL17_Antibody_1_ELISA_051316
  • Poly5230_PURE_CCL17_Antibody_1_ELISA_051316
  • Poly5230_PURE_CCL17_Antibody_2_WB_051316
    Western blot analysis of 50 ng of recombinant human CCL17 (lane 1) and 50 ng of recombinant mouse CCL17 (lane 2) using purified anti-human CCL17 (TARC) (clone Poly5230) antibody.
Cat # Size Price Quantity Avail. Save
523002 100 µg $225
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Description

CCL17 was initially isolated from phytohemagglutinin-stimulated PBMCs. CCL17 is constitutively expressed in the thymus and, under activation, in several cell types. CCL17-mediated recruitment of Th2 cells and CLA+ CD4+ T cells plays a key role in allergic diseases like atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 has been detected in idiopathic pulmonary fibrosis. Also, CCL17 and CCL22 secreted by dendritic cells seem to mediate the recruitment of regulatory T cells to sites of inflammation in patients with chronic hepatitis. CCL17 is upregulated by several combinations of cytokines: TNF-α, IL-4, and IFN-γ in bronchial epithelial cells; TGF-β1, TNF-α, and IFN-γ in human keratinocyte cell lines; and histamine and prostaglandin E2P in immature dendritic cells.    

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human CCL17
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.125-0.5 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CCL17 (TARC) recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified Poly5130 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the  biotinylated Poly5230 antibody (Cat. No. 523003) as the detection antibody and recombinant human CCL17 (TARC) (Cat. No. 573809) as the standard.

Note: For testing human CCL17 (TARC) in serum, plasma or supernatant, BioLegend's ELISA LEGEND MAX™ Kits (Cat. No. 441107 & 441108) are specially developed and recommended.

RRID
AB_2562135 (BioLegend Cat. No. 523002)

Antigen Details

Structure
Chemokine.
Distribution
Upon activation, CCL17 is expressed by PBMC, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.
Function
CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells and plays major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction
T cells, Th2 cells, CLA+ CD4 T cells, T (reg) cells, NK cells, NKT cells, and macrophages.
Ligand Receptor
CCR4.
Cell Type
Monocytes, Dendritic cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Imai T, et al. 1997. J. Biol. Chem. 272:15036.
2. McIlroy A, et al. 2006. Immunology 117:507.
3. Yogo Y, et al. 2009. Respir. Res. 10:80.
4. Stutte S, et al. 2010. Proc. Natl. Acad. Sci. USA 107:8736.
5. Oo YH, et al. 2010. J. Immunol. 184:2886.

Gene ID
6361 View all products for this Gene ID
UniProt
View information about CCL17 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 06/01/2016

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