Purified anti-HDAC5 Antibody

Pricing & Availability
Clone
W15202A (See other available formats)
Other Names
Histone deacetylase 5 (HD5), Antigen NY-CO-9, KIAA0600
Isotype
Rat IgG2b, κ
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Product Citations
publications
W15202A_PURE_HDAC5_Antibody_WB_031519_updated.png
Whole cell extracts (15 µg total protein) from Molt-4, Jurkat, MCF-7, and NIH/3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-HDAC5 Antibody, clone W15202A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
  • W15202A_PURE_HDAC5_Antibody_WB_031519_updated.png
    Whole cell extracts (15 µg total protein) from Molt-4, Jurkat, MCF-7, and NIH/3T3 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti-HDAC5 Antibody, clone W15202A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker.
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693402 100 µg $215
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Description

Histone deacetylases (HDACs) are a family of enzymes capable of regulating acetylation level of histones and non-histone proteins. HDACs have been classified into four classes (class I, II, III, and IV) based on their homology with yeast HDACs. HDAC5 is a member of class IIa HDACs, which contain N-terminal extensions with 14-3-3 binding sites. Phosphorylation of HDAC5 on Ser259 and Ser498 leads to association with 14-3-3 proteins and export from the nucleus. HDAC5 has been suggested to participate in various cellular activities, such as cell proliferation, cell cycle progression, apoptosis, neuronal repair, synoviocyte activation, and myoblast differentiation. Aberrant expression of HDAC5 has been shown to be associated with tumor progression and poor prognosis in cancer patients.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human HDAC5 recombinant protein (320-480 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632835 (BioLegend Cat. No. 693402)

Antigen Details

Structure
1122 amino acids with a predicted molecular weight of 122 kD and observed of approximately 140 kD. Contains a histone deacetylation domain responsible for the removal of acetyl group on the side chain of lysine residue.
Distribution

Nucleus and cytoplasm.

Function
HDAC5 is a class II histone deacetylase and plays important roles in cell proliferation, differentiation, apoptosis, and tumor progression.
Interaction
Interacts with AHRR, BAHD1, BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2, 14-3-3, BCL6, DDIT3/CHOP, GRK5, KDM5B, MYOCD, and EP300.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Xiao H, et al. 2016. Int. J. Cancer 138:2477.
2. Chen S, et al. 2015. Cell Cycle 14:2520.
3. Cho Y, et al. 2013. Cell. 155:894.
4. Kim SS, et al. 2013. Mol. Cell. 52:289.
5. Cho Y, et al. 2012. EMBO J. 31:3063.
6. Watamoto K, et al. 2003. Oncogene. 22:9176.

Gene ID
10014 View all products for this Gene ID
UniProt
View information about HDAC5 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 03/15/2019

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