Purified anti-FUS Antibody

Pricing & Availability
Clone
10F7 (See other available formats)
Other Names
FUS, TLS
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
10F7_PURE_FUS_Antibody_090418
Western blot of purified anti-FUS antibody (clone 10F7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 10F7_PURE_FUS_Antibody_090418
    Western blot of purified anti-FUS antibody (clone 10F7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
855001 25 µg $90
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855002 100 µg $235
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Description

FUS/TLS is an RNA-binding protein that was originally discovered as a fusion protein caused by chromosomal translocation in cancer. FUS/TLS is as an important member of the cellular DNA repair response. The C-terminal end of FUS is involved in protein and RNA binding, while the N-terminal motif of FUS plays a role in transcriptional activation. FUS was also shown to accumulate in the cytoplasm of neurons under hyperosmotic conditions, and FUS mislocalization has been implicated as being the first step toward disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLS/FUS recombinant protein fused with GST tag.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2750473 (BioLegend Cat. No. 855001)
AB_2750474 (BioLegend Cat. No. 855002)

Antigen Details

Structure
FUS is a 526 amino acid protein with an apparent molecular mass of ~ 53 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus and cytosol

Function
FUS/TLS binds to DNA and maintains genomic integrity.
Interaction
Interacts with HDAC1, RNA Polymerase II, FUSIP1, ILF3, PRMT1, RELA, SPI1, and TNPO1.
Cell Type
Astrocytes, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Synaptic Biology
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Efimova AD, et al. 2017. Mol Biol (Mosk) 51:387.
  2. Ederle H, Dormann D. 2017. FEBS Lett. 591:1489.
  3. Guerrero EN, et al. 2016. Prog. Neurobiol. 145-146:78.
Gene ID
2521 View all products for this Gene ID 233908 View all products for this Gene ID 217385 View all products for this Gene ID
UniProt
View information about FUS on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/04/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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