Purified anti-Beclin-1 Antibody

Pricing & Availability
Clone
O93F3 (See other available formats)
Other Names
ATG6 Autophagy Related 6 Homolog, VSP30
Isotype
Mouse IgG1
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Product Citations
publications
O93F3_PURE_Beclin-1_Antibody_060217
Western blot of anti-Beclin-1 antibody (clone O93F3) and isotype-matched IgG1 control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Beclin-1 antibody or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20 µg. The anti-Histone-H3 antibody was used as a loading control.
  • O93F3_PURE_Beclin-1_Antibody_060217
    Western blot of anti-Beclin-1 antibody (clone O93F3) and isotype-matched IgG1 control. Blots were incubated with 5µg/ml (left) or 0.5µg/ml (middle) anti-Beclin-1 antibody or IgG1 isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20 µg. The anti-Histone-H3 antibody was used as a loading control.
Cat # Size Price Quantity Avail. Save
849701 25 µg $100
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849702 100 µg $245
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Description

Beclin-1 plays a central role in autophagy and is involved in autophagosome formation. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Beclin-1 is necessary for the initiation of autophagy, and it has been shown to be reduced in the brains of patients with Alzheimer’s disease. Reduced Beclin-1 levels lead to decreased autophagy, the accumulation of Aβ peptides, and neurodegeneration in mouse models of Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human Beclin-1 protein (NP_003757) produced in HEK293T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Deng X, et al. 2017. Biochim. Biophys. Acta. 1862(3):337-350 (WB).
RRID
AB_2687306 (BioLegend Cat. No. 849701)
AB_2687307 (BioLegend Cat. No. 849702)

Antigen Details

Structure
Beclin-1 is a 450 amino acid protein with a molecular mass of 52 kD.
Distribution

Tissue distribution: Beclin-1 is ubiquitously expressed. </br>Cellular distribution: Cytosol, endoplasmic reticulum, endosome, mitochondria, and nucleus.

Function
Beclin-1 is involved in autophagosome formation in autophagy.
Interaction
Beclin-1 interacts with Bcl-2 and PI3K class III.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References
  1. Cao Y, et al. 2007. Cell. Research 17:839.
Gene ID
8678 View all products for this Gene ID
UniProt
View information about Beclin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05/30/2017

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