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PerCP/Cyanine5.5 anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X45-5-7.1_PerCPCy5.5_CD64_Antibody_FC_1_032613
C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PerCP/Cyanine5.5 (top) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (bottom). Data shown was gated on myeloid population.
  • X45-5-7.1_PerCPCy5.5_CD64_Antibody_FC_1_032613
    C57BL/6 mouse bone marrow cells were stained with CD11b APC and CD64 (clone X54-5/7.1) PerCP/Cyanine5.5 (top) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (bottom). Data shown was gated on myeloid population.
  • X45-5-7.1_PerCPCy5.5_CD64_Antibody_FC_2_032613
Compare all formats See PerCP/Cyanine5.5 spectral data
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139307 25 µg $165
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139308 100 µg $358
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed
  2. Kolter J, et al. 2016. J Immunol. 196: 2733 - 2741. PubMed
  3. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  4. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  5. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  6. Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed
  7. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  8. Baasch S, et al. 2021. Cell. . PubMed
  9. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  10. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  11. De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
  12. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  13. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  14. van Wijck Y, et al. 2018. J Immunol. 200:1570. PubMed
  15. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  16. Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
  17. Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed
  18. Pisu D, et al. 2020. Cell Rep. 30:335. PubMed
  19. Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
  20. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  21. Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
  22. Marinaik CB, et al. 2020. Cell Rep Med. 1:100095. PubMed
RRID
AB_2561962 (BioLegend Cat. No. 139307)
AB_2561963 (BioLegend Cat. No. 139308)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 12/14/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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