PE anti-rat CD2 Antibody

Pricing & Availability
Clone
OX-34 (See other available formats)
Other Names
LFA-2, T11
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
OX-34_PE_011909.jpg
LOU rat splenocytes stained with OX-34 PE
  • OX-34_PE_011909.jpg
    LOU rat splenocytes stained with OX-34 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
201305 50 µg $115
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Description

CD2, also known as LFA-2, is a member of the immunoglobulin gene family with an approximate molecular weight of 45 kD. It is expressed on thymocytes, peripheral T cells, dendritic epidermal T cells, macrophages in the spleen, and NK cells. CD2 can associate with the T cell receptor and participate in T cell activation through adhesion and signal transduction. CD2 binds to CD48, CD58, and CD59. The OX-34 antibody blocks CD48 binding to CD2. It has been reported to partially block T cell activation and inhibit the mixed lymphocyte reaction in vitro. The OX-34 antibody has also been used to deplete peripheral T cells and prevent allograft rejection in vivo.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat T cell blasts from mixed lymphocyte reactions
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation2, partial in vitro blocking of T cell responses3, and in vivo depletion of T cells3.

Application References

(PubMed link indicates BioLegend citation)
  1. Williams AF, et al.. 1987. J. Exp. Med. 165:368.
  2. Jefferies WA, et al.. 1985. J. Exp. Med. 162:117.
  3. Hirahara H, et al.. 1995. Transplantation 59:85.
Product Citations
  1. Shibata T, et al. 2016. Int Immunol. 28: 211 - 222. PubMed
  2. Zhao W, et al. 2019. Nat Commun. 10:124. PubMed
Publication Library
RRID
AB_2073811 (BioLegend Cat. No. 201305)

Antigen Details

Structure
Ig superfamily, approximately 45 kD
Distribution

Thymocytes, T cells, dendritic epidermal T cells, splenic macrophages, NK cells

Function
Adhesion, T cell activation
Ligand/Receptor
CD48, CD59
Cell Type
Macrophages, NK cells, T cells, Thymocytes
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Jefferies WA, et al.. 1985. J. Exp. Med. 162:117.
2. Williams AF, et al.. 1987. J. Exp. Med. 165:368.

Gene ID
497761 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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