PE anti-human IFN-γ R β chain Antibody

Pricing & Availability
2HUB-159 (See other available formats)
Regulatory Status
VI C-111
Other Names
Hamster IgG
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Product Citations
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
  • 2HUB-159_PE_072909.jpg
    Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
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308504 100 tests $304
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IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
soluable extracellular domains of the human IFN-γ R β chain
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308506).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB)
  3. Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB)
  4. Joseph TD, et al. 2001. J. Biol. Chem. 276:47136.
  5. Choi JC, et al. 2007. J. Immunol. 178:1598.
  6. Tjon AS, et al. 2014. J Immunol. 192:5625. PubMed
Product Citations
  1. Tjon A, et al. 2014. J Immunol. 192:5625. PubMed
  2. Kulling PM, et al. 2018. PLoS One. 13:e0193429. PubMed
  3. Ferrando-Martinez S, et al. 2018. J Clin Invest. 128:2089. PubMed
  4. Dela Peña–Ponce MG, et al. 2017. Front Pediatr. 5:15. PubMed
  5. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  6. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
AB_314718 (BioLegend Cat. No. 308504)

Antigen Details

Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD

All cells, except erythrocytes, at low levels

Transducing signals
Biology Area
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.

Gene ID
3459 View all products for this Gene ID
View information about IFN-gamma R beta chain on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08/17/2022

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  • PE anti-human IFN-γ R β chain


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