- AH9R7 (See other available formats)
- Regulatory Status
- Other Names
- CD129, IL-9R
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD129 is known as the 57 kD IL-9 receptor. It is a member of the hematopoietin receptor superfamily. Although the α-chain of the receptor binds IL-9 with high affinity, interaction with the γ-chain (CD132) of the IL-2 receptor is required for signaling. The IL-9 receptor is expressed at low levels on eosinophils, mast cells, macrophages, B lymphocytes, T lymphocytes, and erythroid progenitors. IL-9 receptor binding initiates STAT activation required for the proliferative and anti-apoptotic effects of this cytokine. In humans, signals from the IL-9 receptor appear to be critical for intrathymic T cell development. IL-9 binding has been shown to increase IL-5 receptor expression and promote survival in human eosinophils.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition the binding of IL-9 to the high affinity a-chain of the human IL-9 receptor1,2, and ELISA for detection of soluble IL-9R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 310404) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310408).
(PubMed link indicates BioLegend citation)
- De Smedt M, et al. 2000. J. Immunol. 164:1761.
- Pilette C, et al. 2002. J. Immunol. 168:4103.
- Demoulin JB, et al.1996. Mol. Cell. Biol. 16 (9):4710.
- Product Citations
AB_314816 (BioLegend Cat. No. 310403)
AB_314817 (BioLegend Cat. No. 310404)
- Cytokine receptor family, type I membrane protein or soluble, associate with common γ chain(CD132), 57 kD
Eosinophils, mast, thymocytes, erythroid, myeloid progenitors, macrophages
- Activates STAT; critical for intrathymic T cell development
- Cell Type
- Eosinophils, Erythrocytes, Hematopoietic stem and progenitors, Macrophages, Mast cells, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Demoulin JB, et al.1996. Mol. Cell. Biol. 16:4710.
- Gene ID
- 3581 View all products for this Gene ID
- View information about CD129 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
|PE anti-human CD129 (IL-9 R)||AH9R7||FC|
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