ELISA MAX™ Deluxe Set Mouse IL-9

Pricing & Availability
Regulatory Status
Other Names
P40, MEA (mast cell growth enhancing activity), MCGF (Mast cell growth factor), TCGF-3 (T cell growth factor-3
Ave. Rating
Submit a Review
Product Citations
  • IL-9_Mouse_ELISA_MAX_Deluxe_063016
Cat # Size Price Quantity Check Availability Save
442704 5 plates $347
Check Availability

Need larger quantities of this item?
Request Bulk Quote

Please contact our sales representative for bulk pricing quotations.

Mouse Interleukin 9 (IL-9) is a 144 amino acid, 16.1 kD member of the TH2 cytokine family. Originally identified as a mast cell growth factor, IL-9 has been identified to promote IL-6 and protease production in mast cells that can contribute to the severity of hypersensitivity disorders. These characteristics, among others, have made IL-9 a molecule of interest in studies of hypersensitivity disorders, such as asthma.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A mouse IL-9 specific rat monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-9 binds to the immobilized capture antibody.  Next, a biotinylated goat polyclonal anti-mouse IL-9 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-9 present in the sample.  Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-9 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-9 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-9 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity


As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Heim L, et al. 2022. Front Immunol. 13:859738. PubMed
1.8 pg/mL
Standard Range
3.2 - 200 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered.
  • Wash Buffer: PBS + 0.05% Tween-20, BioLegend Cat. No. 421601 is recommended.
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended.
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Gene ID
16198 View all products for this Gene ID
View information about IL-9 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 09/07/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587


Remember me
Forgot your password? Reset Password
Request an Account