MojoSort™ Human CD19 Selection Kit

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MojoSort_Human_CD19_Selection_Kit_061318
Single cell suspension from human PBMCs was prepared for positive selection of CD19+ cells using the MojoSort™ Human CD19 Selection Kit. Cells were stained with anti-human CD19 (clone HIB19) PE and anti-human CD3 (clone UCHT1) APC. Dead cells were excluded by 7-AAD.
  • MojoSort_Human_CD19_Selection_Kit_061318
    Single cell suspension from human PBMCs was prepared for positive selection of CD19+ cells using the MojoSort™ Human CD19 Selection Kit. Cells were stained with anti-human CD19 (clone HIB19) PE and anti-human CD3 (clone UCHT1) APC. Dead cells were excluded by 7-AAD.
Cat # Size Price Quantity Avail. Save
480105 20 tests $150
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480106 200 tests $550
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Description

Human CD19+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-human CD19 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD19+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat# 480105:

  • 200 µl of Biotin anti-human CD19 antibody (clone SJ25C1)
  • 200 µl Streptavidin Nanobeads
     

 For Cat# 480106:

  • 2 vials of 1ml of Biotin anti-human CD19 antibody (clone SJ25C1) each
  • 2 vials of 1ml of Streptavidin Nanobeads each

Product Details

Reactivity
Human
Formulation
Antibody: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2.

Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested

Recommended Usage

10 µl of Biotin anti-human CD19 antibody for 1 x 107 cells in 100 µL of buffer.

10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for the positive selection or depletion of human CD19+ cells from peripheral blood mononuclear cells (PBMCs).

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
 

Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 8X

Antigen Details

Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD19 on UniProt.org

Related FAQs

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems.  Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

Can your magnetic particles be sterile filtered?

Yes, they can be sterile filtered as the particles are smaller than 0.22 µm.

Is there a way to detach your magnetic particles from the cell surface?

No, not currently.  We have found that cells are functional without the need to detach the magnetic Nanobeads.

What is the size of your magnetic particles?

The average diameter is approximately 130 nm.

Go To Top Version: 1    Revision Date: 06/14/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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