- Other Names
- Multiplex, flow cytometry, CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, IL-1β
- Ave. Rating
- Submit a Review
- Product Citations
The LEGENDplex™ Mouse Macrophage/Microglia Panel Standard contains 13 mouse proteins, including CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, and IL-1β. The beads are intended for use with the following in Mix and Match assays:
Mouse Macrophage/Microglia Panel
740851 LEGENDplex™ Mouse Macrophage/Microglia Panel Detection Antibodies
740853 LEGENDplex™ Mouse CXCL1 (KC) Capture Bead A4, 13X
740854 LEGENDplex™ Mouse Free Active TGF-β1 Capture Bead A5, 13X
740855 LEGENDplex™ Mouse IL-18 Capture Bead A6, 13X
740856 LEGENDplex™ Mouse IL-23 Capture Bead A7, 13X
740857 LEGENDplex™ Mouse CCL22 (MDC) Capture Bead A8, 13X
740858 LEGENDplex™ Mouse IL-10 Capture Bead A10, 13X
740859 LEGENDplex™ Mouse IL-12p70 Capture Bead B2, 13X
740860 LEGENDplex™ Mouse IL-6 Capture Bead B3, 13X
740861 LEGENDplex™ Mouse TNF-α Capture Bead B4, 13X
740862 LEGENDplex™ Mouse G-CSF Capture Bead B5, 13X
740863 LEGENDplex™ Mouse CCL17 (TARC) Capture Bead B6, 13X
740864 LEGENDplex™ Mouse IL-12p40 Capture Bead B7, 13X
740865 LEGENDplex™ Mouse IL-1β Capture Bead B9, 13X
740373 LEGENDplex™ Buffer Set B
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-Bottom Plate
- Biology Area
- Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.