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- Ig Isotyping Panel
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The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel Standard can be used together with the following Mix and Match Capture Beads and Mouse Immunoglobulin Isotyping Panel Detection Antibodies to offer flexible customization within the panel (6-plex):
740495 (LEGENDplex™ Mouse Immunoglobulin Isotyping Panel Detection Antibodies)
740375 (LEGENDplex™ Buffer Set D)
740496 (LEGENDplex™ Mouse IgG1 Capture Bead A4, 13X)
740497 (LEGENDplex™ Mouse IgG2a Capture Bead A5, 13X)
740498 (LEGENDplex™ Mouse IgG2b Capture Bead B4, 13X)
740499 (LEGENDplex™ Mouse IgG3 Capture Bead A7, 13X)
740500 (LEGENDplex™ Mouse IgA Capture Bead B2, 13X)
740501 (LEGENDplex™ Mouse IgM Capture Bead B3, 13X)
740377 or 740379 (Filter Plate or V-bottom Plate for LEGENDplex™ Assay)
The LEGENDplex™ Mouse Immunoglobulin Isotyping Panel is a multiplex bead-based assay, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 6 mouse proteins, including IgG1, IgG2a, IgG2b, IgG3, IgA, IgM. This assay panel provides high detection sensitivities and broad dynamic ranges. The panel has been validated by detecting expected changes in biological samples.
- Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.