- Other Names
- Multiplex assay, Flow cytometry, Human Immune Checkpoint Panel, Mix and Match
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- Product Citations
The LEGENDplex™ Buffer Set M contains: Wash Buffer, Assay Buffer, SA-PE, Matrix A1, Setup Beads 1, 2, 3, Plate Sealers, and Software Dongle. LEGENDplexTM Buffer Set M is used in HU Immune Checkpoint Mix and Match Subpanels with the following products:
HU Immune Checkpoint Panel 1
740868 LEGENDplex™ HU Immune Checkpoint Panel 1 Detection Antibodies
740869 LEGENDplex™ HU Immune Checkpoint Panel 1 Standard
740870 LEGENDplex™ Human sCD25 (IL-Ra) Capture Bead A4, 13X
740872 LEGENDplex™ Human 4-1BB Capture Bead A6, 13X
740873 LEGENDplex™ Human sCD27 Capture Bead A7, 13X
740874 LEGENDplex™ Human B7.2 (CD86) Capture Bead A8, 13X
740875 LEGENDplex™ Human Free Active TGF-β1 Capture Bead A10, 13X
740876 LEGENDplex™ Human CTLA-4 Capture Bead B2, 13X
740877 LEGENDplex™ Human PD-L1 Capture Bead B3, 13X
740878 LEGENDplex™ Human PD-L2 Capture Bead B4, 13X
740879 LEGENDplex™ Human PD-1 Capture Bead B5, 13X
740880 LEGENDplex™ Human Tim-3 Capture Bead B6, 13X
740881 LEGENDplex™ Human LAG-3 Capture Bead B7, 13X
740882 LEGENDplex™ Human Galectin-9 Capture Bead B9, 13X
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-bottom Plate
- Gene ID
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.