ELISA MAX™ Deluxe Set Human CCL5 (RANTES)

Pricing & Availability
Other Names
D17S136E, SCYA5, SISd, TCP228
Ave. Rating
1 reviews
Product Citations
publications
CCL5_Human_ELISA_Kit_Deluxe_121313
  • CCL5_Human_ELISA_Kit_Deluxe_121313
Cat # Size Price Quantity Save
440804 5 plates $295
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Description

The 10- and 20-plate sizes of this assay have been discontinued. The assay will remain available for purchase under the 5-plate catalog number, but will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

CCL5, also known as RANTES, is a member of the CC family of chemokines. The precursor of CCL5 contains 91 amino acids, with an N-terminal signal peptide of 23 amino acids which is cleaved to generate the 68 amino acids mature protein (7.8 kD). Human mature CCL5 shares 82% - 90% amino acid seqeuence identity with equine, porcine, bovine, mouse, rat and canine CCL5.

CCL5 is expressed by T cells and monocytes/macrophages. It plays an active role in recruiting leukocytes into inflammatory sites through receptors CCR1, CCR3, CCR4, and CCR5. CCL5 induces the proliferation and activation of certain natural killer (NK) cells. It also inhibits HIV-1 entry and infection. In addition, CCL5 plays a crucial role in the migration and metastasis of cancer cells. Increased CCL5 levels are found in melanoma, breast, cervical, prostate, gastric, and pancreatic cancers.


Both the ELISA MAX™ Set Standard and ELISA MAX™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human CCL5 (RANTES) ELISA MAX™ Capture Antibody (200X)
  • Human CCL5 (RANTES) ELISA MAX™ Detection Antibody (200X)
  • Human CCL5 (RANTES) Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Patsouras M, et al. 2015. J Autoimmun. Available online 15 August 2015. PubMed
  2. Chabanon RM, et al. 2019. J Clin Invest. 129:1211. PubMed
Publication Library
Sensitivity
3 pg/mL
Standard Range
7.8-500 pg/ml
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
T cells, monocytes/macrophages, mesangial cells, platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
6352 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe sets no longer come with plates, but Coating Buffer and Assay Diluent (for blocking and dilutions) are included in the ELISA MAX™ Deluxe Sets. Plates can be ordered separately (Cat. No. 423501), and instructions on coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 04/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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