HRP anti-SNAP-25 Antibody

Pricing & Availability
Clone
SMI 81 (See other available formats)
Other Names
Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
Isotype
Mouse IgG1, κ
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Product Citations
publications
SMI-81_HRP_SNAP-25_Antibody_WB_121217
Western blot of HRP anti-SNAP-25 antibody (clone SMI 81). Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of rat brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. HRP SNAP-25 was visualized using chemiluminescence detection.
  • SMI-81_HRP_SNAP-25_Antibody_WB_121217
    Western blot of HRP anti-SNAP-25 antibody (clone SMI 81). Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of rat brain lysate; Lane 3: 20 µg of mouse brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. HRP SNAP-25 was visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
836305 25 µg $105
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836306 100 µg $265
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Description

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length, unaltered SNAP-25.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC)
  2. Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed
  3. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
  4. Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed
  5. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
  6. Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed
  7. Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed
  8. Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
RRID
AB_2721609 (BioLegend Cat. No. 836305)
AB_2721610 (BioLegend Cat. No. 836306)

Antigen Details

Structure
SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Cellular distribution: Cytosol, golgi apparatus, and cell membrane.

Function
SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction
In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References

1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23.
2. Nagy G, et al. 2004. Neuron. 41(3):417–29.

Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 12/13/2017

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