HRP anti-Beclin-1 Antibody

Pricing & Availability
Clone
O93F3 (See other available formats)
Other Names
ATG6 Autophagy Related 6 Homolog, VSP30
Isotype
Mouse IgG1
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Product Citations
publications
O93F3_HRP_Beclin-1_Antibody_022118
Western blot of HRP anti-Beclin-1 antibody (clone O93F3). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. The bands were visualized using chemiluminescence detection.
  • O93F3_HRP_Beclin-1_Antibody_022118
    Western blot of HRP anti-Beclin-1 antibody (clone O93F3). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. The bands were visualized using chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
849703 25 µg $130
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849704 100 µg $275
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Description

Beclin-1 plays a central role in autophagy and is involved in autophagosome formation. It acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Beclin-1 is necessary for the initiation of autophagy, and it has been shown to be reduced in the brains of patients with Alzheimer’s disease. Reduced Beclin-1 levels lead to decreased autophagy, the accumulation of Aβ peptides, and neurodegeneration in mouse models of Alzheimer's disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant human Beclin-1 protein (NP_003757) produced in HEK293T cells
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Deng X, et al. 2017. Biochim. Biophys. Acta. 1862(3):337-350 (WB).
RRID
AB_2728575 (BioLegend Cat. No. 849703)
AB_2728576 (BioLegend Cat. No. 849704)

Antigen Details

Structure
Beclin-1 is a 450 amino acid protein with a molecular mass of 52 kD.
Distribution

Tissue distribution: Beclin-1 is ubiquitously expressed.
Cellular distribution: Cytosol, endoplasmic reticulum, endosome, mitochondria, and nucleus.

Function
Beclin-1 is involved in autophagosome formation in autophagy.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References
  1. Cao Y, et al. 2007. Cell. Research 17:839 
Gene ID
8678 View all products for this Gene ID
UniProt
View information about Beclin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/22/2018

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