Flash Phalloidin™ Red 594

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Other Names
Phalloidin
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Flash_Phalloidin_Red_594_042216
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with Flash Phalloidin™ Red 594 (5 µl of the stock solution in 200 µL of 5% FBS) for 20 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue).
  • Flash_Phalloidin_Red_594_042216
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the cells were stained with Flash Phalloidin™ Red 594 (5 µl of the stock solution in 200 µL of 5% FBS) for 20 minutes at 4°C in the dark. The nuclei were then counterstained with DAPI (blue).
  • Flash_Phalloidin_Red_594_IHC_010217
    C57 mouse frozen cerebellum tissue was fixed with 4% paraformaldehyde (PFA) for ten minutes then permeabilized with 0.5% Triton X-100 for ten minutes and blocked by 5% FBS for 30 minutes. Then, the tissue was stained with Flash Phalloidin™ Red 594 (red, 25 µL of the stock solution in 1 mL of PBS) for 30 minutes at 4°C in the dark. The tissue was then counterstained with DAPI (blue). The image was captured by 20X objective.
See Flash Phalloidin™ Red 594 spectral data See high resolution IF/IHC-F data...
Cat # Size Price Quantity Avail. Save
424203 300 units $295
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Description

Molecular Mass 1535.79 g/mol.  Flash Phalloidin™ Red 594 excites maximally at 590 nm and emits maximally at 611 nm. 

Phalloidin is a bicyclic peptide that can be found naturally in the death cap mushroom.  This molecule is considered to bind so tightly to F-actin that when ingested by an organism, it will prevent the depolymerization of the actin polymeric filaments which leads to cellular toxicity.  In cell imaging, this is a very useful probe for imaging and stabilizing filamentous F-actin in fixed and permeabilized cells, providing structural and volumetric context to the cell.  Phallotoxins are conjugated to a wide array of fluorophores to enable their use in multicolor microscopy.

Product Details
Technical data sheet

Product Details

Preparation
Flash Phalloidin™ Red 594 is lyophilized. Reconstitute with 1.5mL of methanol to make a stock solution of 300 units.
Storage & Handling
Store Flash Phalloidin™ Red 594 at -20°C, protected from light.
Application

IF - Quality tested
IHC-F - Validated

Recommended Usage

Reconstitute the Flash Phalloidin™ Red 594 with 1.5mL of methanol to make 300 units. Then prepare a working concentration by diluting 1:20 - 1:100 of stock in 1X PBS. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
  1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial.
  2. Fix cultured cells with 1% - 4% paraformaldehyde (PFA) for 10 minutes at room temperature.
  3. Wash the cells two times with 1X PBS.
  4. Permeabilize the cells with 0.5% Triton X-100 for 10 minutes at room temperature or at 4°C.
  5. Wash the cells two times with 1X PBS.
  6. Block cells with 5% fetal bovine serum for 30 minutes at room temperature.
  7. Prepare the working solution by diluting Flash Phalloidin™ Red 594 1:20 - 1:100 in 1X PBS.
  8. Stain the cells with diluted solution for 20 minutes at room temperature in the dark protected from light.
  9. Mount the slides with an antifade mounting media and image the slides.

Antigen Details

Distribution

Cytoskeleton.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience
Gene ID
NA

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Go To Top Version: 3    Revision Date: 01/02/2017

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