FITC anti-human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56)

Pricing & Availability
Clone
UCHT1, HCD14, HIB19, 2H7, HCD56 (See other available formats)
Isotype
Mouse IgG1, κ; Mouse IgG2b, κ
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Product Citations
publications
hlin2cocktail_fitc_1_083110
Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
  • hlin2cocktail_fitc_1_083110
    Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
  • hlin2cocktail_fitc_2_083110
    Human peripheral blood co-stained with CD11c APC and CD16 PE, gated on the lineage negative/dim and HLA-DR+ population
See FITC spectral data
Cat # Size Price Quantity Avail. Save
348701 50 tests $495
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Description

This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)

Antigen Details

Distribution
CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.
Cell Type
T cells, Monocytes, Macrophages, Neutrophils, Eosinophils, Mesenchymal Stem Cells, Dendritic cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey.
2. Olweus J, et al. 1997. P. Natl. Acad. Sci. USA 94:12551.
3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York.
4. Markowicz S, et al. 1990. J. Clin. Invest. 85:955.
5. Freudenthal P, et al. 1990. P. Natl. Acad. Sci. USA 87:7698.
6. Macdonald K, et al. 2002. Blood 100:4512.
7. Piccioli D, et al. 2007. Blood 109:5371.

Gene ID
NA
UniProt
View information about Lineage on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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