FITC anti-human CD77 Antibody

Pricing & Availability
5B5 (See other available formats)
Regulatory Status
VI CD77.5
Other Names
globotriaosylcermamide (Gb3), Burkitt's lymphoma associated antigen (BLA), cermadie trihexoside (CTH), and Pk blood group antigen
Mouse IgM, κ
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Product Citations
Human B-cell Burkitt's lymphoma cell line Daudi was stained with CD77 (clone 5B5) FITC (filled histogram) or mouse IgM, κ FITC isotype control (open histogram).
  • 5B5_FITC_CD77_Antibody_FC_052413
    Human B-cell Burkitt's lymphoma cell line Daudi was stained with CD77 (clone 5B5) FITC (filled histogram) or mouse IgM, κ FITC isotype control (open histogram).
See FITC spectral data
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357103 25 tests $101
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357104 100 tests $252
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CD77, also known as Gb3, is a type II membrane protein. It was found specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center B lymphocytes. CD77 has been described as a receptor for Shiga Toxin and Verotoxin 1 (VT1), which is produced by Shigella dysenteriae and some strains of E. coli. It is used as a marker for germinal center stage B-cells. CD19 has been reported as a ligand for D77 and their interaction functions in adhesion and signal transduction at a specific stage of B cell development. Studies have shown that cross-linking CD77 induces apoptosis in some B cell lines.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human peripheral blood mononuclear cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for relevant formats) include:  Western blotting1 and immunohistochemical staining of frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Fyfe G, et al. 1987. J. Immunol. 139:2187. (WB, IHC)
Product Citations
  1. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
AB_2562160 (BioLegend Cat. No. 357103)
AB_2562161 (BioLegend Cat. No. 357104)

Antigen Details

Type II membrane protein

Burkitt's lymphoma cells, a subset of germinal center B lymphocytes

Involved in cell adhesion and signaling transduction by binding CD19
Receptor of Shiga Toxin and Verotoxin 1 (VT1) and CD19
Cell Type
B cells, Lymphocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tétaud C, 2003. J. Biol. Chem. 278:45200.
2. Maloney MD, 1994. J. Exp. Med. 1994 180:191.
3. Mangeney M, et al. 1991. Eur. J. Immunol. 21:1131.
4. Mangeney M, et al. 1993. Cancer Res. 53:5314.

Gene ID
53947 View all products for this Gene ID
View information about CD77 on

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Go To Top Version: 2    Revision Date: 01/27/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human CD77

  • FITC anti-human CD77


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