ELISA MAX™ Deluxe Set Human Uncarboxylated Osteocalcin

Pricing & Availability
Regulatory Status
RUO
Other Names
Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, uOCN, uOC, 3XGLU
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publications
ELISA-MAX_Deluxe_Human_Uncarboxylated_Osteocalcin
  • ELISA-MAX_Deluxe_Human_Uncarboxylated_Osteocalcin
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446704 5 plates $358
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Description

Osteocalcin is the bone’s most abundant non-collagenous protein. It is produced by osteoblasts as a 49 amino acid protein with 3 glutamine acid residues (Glu 17, 21, and 24) that are gamma carboxylated in a vitamin K-dependent manner. The fully carboxylated protein has a high affinity for the bone extracellular matrix. Bone resorption results in a decrease in pH, causing the decarboxylation of osteocalcin. This generates partially decarboxylated (undercarboxylated), or fully decarboxylated (uncarboxylated) osteocalcin. These forms of osteocalcin have decreased affinity for the bone extracellular matrix and are released into the bloodstream, allowing it to bind the receptor GPCR6a, acting as hormones promoting insulin sensitivity, glucose tolerance, and testosterone biosynthesis. In patients with diabetes, reduced serum levels of osteocalcin are negatively correlated with obesity and insulin resistance.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human uncarboxylated osteocalcin specific monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and uncarboxylated osteocalcin binds to the immobilized capture antibody. Next, a biotinylated anti-human osteocalcin (uncarboxylated) detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of uncarboxylated osteocalcin present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human Uncarboxylated OCN ELISA MAX™ Capture Antibody (200X)
  • Human Uncarboxylated OCN ELISA MAX™ Detection Antibody (200X)
  • Human Uncarboxylated OCN Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
12.6 ± 1.7 pg/mL
Standard Range
23.4 - 1500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS +
    0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid
    solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4,
    0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm
    filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Osteoblasts
Molecular Family
Hormones
Gene ID
NA
UniProt
View information about Uncarboxylated Osteocalcin on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 08/25/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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