ELISA Assay Diluent (5X)

Pricing & Availability
Regulatory Status
Other Names
ELISA assay diluent, ELISA diluent
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Product Citations
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421203 100 mL $71
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ELISA Assay Diluent is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (specifically Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.

Product Details
Technical data sheet

Product Details

Storage & Handling
This solution can be stored unopened between 2°C and 8°C. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20°C (frost-free) or -70°C for long term storage.

Warm ELISA reagents to room temperature (18-25°C) before use.


Recommended Usage

Prior to use, dilute the ELISA Assay Diluent (5X) in PBS, pH 7.4.

PBS recipe: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl. Add deionized water to 1.0 L, filter through 0.2 µm filter, pH to 7.4

Application References

(PubMed link indicates BioLegend citation)
  1. Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed
Product Citations
  1. Maizels K 2014. Nucleic Acids Res. 42:108. PubMed

Antigen Details

Gene ID

Related FAQs

I ran out of assay diluent provided with ELISA MAX™ Deluxe set.

You can order more assay diluent using Cat. No. 421203.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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