Direct-Blot™ HRP anti-NF-κB p50 Antibody

Pricing & Availability
Clone
4D1 (See other available formats)
Other Names
NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
4D1_DBHRP_NF-kB-p50_Antibody_070918
Total cell lysates (15 µg protein) from NIH3T3 (lane 1), Jurkat (lane 2) and HeLa cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:4000 diluted Direct-Blot™ HRP anti-NF-κB p50 Antibody, clone 4D1 (upper panel) or 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (lower panel) as a loading control. Proteins were visualized by chemiluminescence detection. Lane M: Molecular weight ladder.
  • 4D1_DBHRP_NF-kB-p50_Antibody_070918
    Total cell lysates (15 µg protein) from NIH3T3 (lane 1), Jurkat (lane 2) and HeLa cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:4000 diluted Direct-Blot™ HRP anti-NF-κB p50 Antibody, clone 4D1 (upper panel) or 1:4000 Direct-Blot™ HRP anti-GAPDH antibody, clone W17079A (lower panel) as a loading control. Proteins were visualized by chemiluminescence detection. Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
616707 100 µl $290
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Description

NF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant full-length human NF-κB p50
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000 - 1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed
  2. Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed
  3. Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.
RRID
AB_2566705 (BioLegend Cat. No. 616707)

Antigen Details

Structure
Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
Distribution

Ubiquitously expressed

Function
A transcription factor, originally identified as an activator of kappa light chain in B cells
Interaction
Interacts with NF-κB p65 and IκB
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).

Regulation
NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p50 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 07/09/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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