Purified anti-NF-κB p50 Antibody

Pricing & Availability
Clone
4D1 (See other available formats)
Other Names
NF-κB p50, nuclear factor kappa light chain enhancer in B cells p50, NF-κB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
4D1_Pure_NF-kB-p50_Antibody_070918
Total cell lysates (15 µg protein) from Jurkat (Lane 1) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.025 µg/mL (1:20,000 dilution) Purified anti-NF-κB p50 Antibody, clone 4D1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-NF-κB p50 Antibody , or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder.
  • 4D1_Pure_NF-kB-p50_Antibody_070918
    Total cell lysates (15 µg protein) from Jurkat (Lane 1) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.025 µg/mL (1:20,000 dilution) Purified anti-NF-κB p50 Antibody, clone 4D1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-NF-κB p50 Antibody , or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1 (lower panel) as a loading control. Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
616701 25 µg $85
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616702 100 µg $185
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Description

NF-κB p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. NF-κB is a transcription factor, originally identified as an activator of kappa light chain in B cells. NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation. This 4D1 monoclonal antibody recognizes p105 (precursor of p50) and p50 (active form). 4D1 has been validated for Western blotting.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
recombinant full-length human NF-κB p50
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store between 2°C and 8°C.
Application

WB - Quality tested
ICC - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1 on the Amnis ImageStream 100 Imaging Flow Cytometer.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References

(PubMed link indicates BioLegend citation)
  1. Huang F, et al. 2007. J. Immunol. 179:6504. (IF) PubMed
  2. Cao YA, et al. 2008. J. Biol. Chem. 283:15309. PubMed
  3. Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed.
Product Citations
  1. Huang F, et al. 2007. J Immunol. 179:6504. PubMed
  2. Kao C, et al. 2008. J Biol Chem. 283:15309. PubMed
  3. Lin D, et al. 2012. J Biol Chem. 287:4299. PubMed
Publication Library
RRID
AB_315855 (BioLegend Cat. No. 616701)
AB_315856 (BioLegend Cat. No. 616702)

Antigen Details

Structure
Member of the Rel/dorsal family. NF-κB p50 is an active form, derived from NF-κB p105, a precursor form.
Distribution

Ubiquitously expressed

Function
A transcription factor, originally identified as an activator of kappa light chain in B cells
Interaction
Interacts with NF-κB p65 and IκB
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Baldwin AS, et al. 1996. Annu. Rev. Immunol. 14:649 (review).
2. Chen F, et al. 1999. Clin. Chem. 45:7 (review).

Regulation
NF-κB p50, together with another NF-κB subunit p65, binds to IκB inhibitor in cytoplasm as an inactive form. Phosphorylation of IκB releases NF-κB p50 and p65. Active form of NF-κB p50 and p65 then translocate to the nucleus and regulate target gene expression. Bacterial products and stimulation of a variety of receptors lead to NF-κB signal pathway activation.
Gene ID
4790 View all products for this Gene ID
UniProt
View information about NF-kappaB p50 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 07/09/2018

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