Loading...

Brilliant Violet 711™ anti-human CD200 (OX2) Antibody

Pricing & Availability
Clone
OX-104 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70655
Other Names
OX-2, OX2
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
OX-104_BV711_CD200_Antibody_071117
Human peripheral blood lymphocytes were stained with CD200 (clone OX-104) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
  • OX-104_BV711_CD200_Antibody_071117
    Human peripheral blood lymphocytes were stained with CD200 (clone OX-104) Brilliant Violet 711™ (filled histogram) or mouse IgG1, κ Brilliant Violet 711™ isotype control (open histogram).
Compare all formats See Brilliant Violet 711™ spectral data
Cat # Size Price Quantity Check Availability Save
329222 25 tests $204
Check Availability


Need larger quantities of this item?
Request Bulk Quote
329223 100 tests $435
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD200, also known as OX2, is a member of the immunoglobulin superfamily (IgSF). It is a monomorphic cell surface glycoprotein that is expressed on thymocytes, neurons, endothelium, follicular dendritic cells in all lymphoid organs, a subset of CD34+ progenitor cells, and at low levels on some smooth muscle and B lymphocytes. It is not expressed on NK cells, monocytes, granulocytes, or platelets. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. The interaction between CD200 (OX2) and CD200 receptor (OX2R) system is of importance in the control of macrophage and granulocyte activation, which may contribute to pathways that suppress and limit macrophage induced inflammatory damage in tissue.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of formalin-fixed paraffin-embedded sections1 and acetone-fixed frozen sections2, and blocking of CD200 interaction with CD200R.

Application References

(PubMed link indicates BioLegend citation)
  1. Patel GK, et al. 2012. J. Invest. Dermatol. 132:401. (IHC)
  2. Wright GJ, et al. 2001. Immunology 102:173. (IHC)
  3. Foster-Cuevas M, et al. 2004. J. Virol. 78:7667. (FC)
Product Citations
  1. Henry GH et al. 2018. Cell reports. 25(12):3530-3542 . PubMed
  2. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
RRID
AB_2715823 (BioLegend Cat. No. 329222)
AB_2715824 (BioLegend Cat. No. 329223)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

T cells, neurons, endothelium

Function
Costimulates T cell proliferation
Receptors
CD200R
Cell Type
Endothelial cells, Neurons, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Wright GJ, et al. 2001. Immunol. 102:173.
2. Foster-Cuevas M, et al. 2004. J. Virol. 78:7667.
3. Mason D, et al. 2002. ed. Leukocyte Typing VII. New York:Oxford Univ. Press.
4. Broderick C, et al. 2002. Am. J. Pathol. 161:1669.

Regulation
Induces a downregulation of macrophage activity
Gene ID
4345 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 07/11/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD200 (OX2)

  • Biotin anti-human CD200 (OX2)

  • PE anti-human CD200 (OX2)

  • APC anti-human CD200 (OX2)

  • Brilliant Violet 421™ anti-human CD200 (OX2)

  • PE/Cyanine7 anti-human CD200 (OX2)

  • Alexa Fluor® 647 anti-human CD200 (OX2)

  • PerCP/Cyanine5.5 anti-human CD200 (OX2)

  • Brilliant Violet 605™ anti-human CD200 (OX2)

  • Purified anti-human CD200 (OX2) (Maxpar® Ready)

  • APC/Cyanine7 anti-human CD200 (OX2)

  • Brilliant Violet 711™ anti-human CD200 (OX2)

  • APC/Fire™ 750 anti-human CD200 (OX2)

  • Ultra-LEAF™ Purified anti-human CD200 (OX2)

  • TotalSeq™-C0447 anti-human CD200 (OX2)

  • TotalSeq™-D0447 anti-human CD200 (OX2)

  • TotalSeq™-A0447 anti-human CD200 (OX2)

  • TotalSeq™-B0447 anti-human CD200 (OX2)

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account