Brilliant Violet 421™ anti-STAT6 Phospho (Tyr641) Antibody

Pricing & Availability
Clone
A15137E (See other available formats)
Other Names
Signal transducer and activator of transcription 6, IL-4 Stat
Isotype
Mouse IgG1, κ
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Product Citations
publications
A15137E_BV421_STAT6-P-Tyr641_Antibody_012120
Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with STAT6 Phospho (Tyr 641) (clone A15137E) Brilliant Violet 421™.
  • A15137E_BV421_STAT6-P-Tyr641_Antibody_012120
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) IL-4 for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with STAT6 Phospho (Tyr 641) (clone A15137E) Brilliant Violet 421™.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
686019 25 tests $185
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686020 100 tests $395
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Description

STAT6 is a member of the signal transducer and activator of transcription (STAT) family, activating gene expression in response to IL-4 and IL-13 stimulation. Upon cytokine stimulation, the receptor is phosphorylated by the associated Janus Kinases (Jak), followed by recruiting cytoplasmic STAT6. The Tyr641 residue of STAT6 is, in turn, phosphorylated by Jak. Phosphorylated STAT6 forms homodimers, translocates to the nucleus, and regulates transcription of target genes. STAT6 plays crucial roles in differentiation of T helper 2 (Th2) cells, class switch of immunoglobulins in B cells, expression of cell surface markers such as MHC class II, and the development of allergic inflammation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT6 peptide phosphorylated at Tyr 641
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Human STAT6 has three isoforms; the molecular weights are 94, 82 and 74kD. The immunogen (phosphorylated peptide) is shared by these three isoforms. This antibody recognizes STAT6 Phospho (Tyr641) in all three isoforms. The predominant band detected is at 94 kD.

Clone A15137E does not react with mouse.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2832847 (BioLegend Cat. No. 686019)
AB_2832848 (BioLegend Cat. No. 686020)

Antigen Details

Structure
847 amino acids with a predicted molecular weight of 94 kD. Contains a SH2 domain responsible for phosphor-Tyrosine binding and dimerization.
Distribution

Cytoplasm; translocates to the nucleus when phosphorylated.

Function
STAT6 is a transcription factor, mediating IL-4 and IL-13 signaling. While STAT6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following phosphorylation by Janus kinases, STAT6 translocates to the nucleus where it regulates gene transcription.
Interaction
STAT6 interacts with CBP/p300 and NCOA1. Forms a homodimer or a heterodimer with other STAT family members.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Goenka S, et al. 2011. Immunol. Res. 50:87.
2. Wurster AL, et al. 2000. Oncogene 19:2577.
3. Akira S. 1999. Stem Cells 17:138.
4. Zamorano J, et al. 2005. J. Immunol. 174:2843.
5. David M, et al. 2001. Oncogene 20:6660.
6. Takeda K, et al. 1996. Nature 380:627.

Gene ID
6778 View all products for this Gene ID
UniProt
View information about STAT6 Phospho Tyr641 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 01/21/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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