- BL1a-89 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF) (il-1a,il1a)
- Rat IgG1, κ
- Ave. Rating
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- Product Citations
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IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses.Product Details
- Antibody Type
- Host Species
- E. coli - expressed, recombinant mouse IL-1α
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection - Quality tested
WB, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.05 - 0.2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-1α recobinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated BL1a-89 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified BL1a-72 antibody (Cat. No. 512402) or purified ALF-161 antibody (Cat. Nos. 503202 & 503206) as the capture antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.
- Product Citations
AB_2295879 (BioLegend Cat. No. 512503)
AB_2124220 (BioLegend Cat. No. 512504)
- Cytokine; 17.4 kD (Mammalian)
- Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
- Cell Sources
- Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
- Cell Targets
- B cells, T cells, monocytes, fibroblasts
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Gene ID
- 16175 View all products for this Gene ID
- View information about IL-1alpha on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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