Biotin anti-human MMP-9 Antibody

Pricing & Availability
M2108F07 (See other available formats)
Regulatory Status
Other Names
Gelatinase B, Type IV collagenase, 92 kD gelatinase, Type V collagenase, GELB, CLG4B, MMP-9, MANDP2
Mouse IgG2a, κ
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Product Citations
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532101 100 µg $265
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MMP9, or MMP-9, is a member of the matrix metalloprotease family of proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Proteolytic activities of MMPs play an important role in vascular remodeling, cellular migration, and the processing of ECM proteins and adhesion molecules. MMP9 consists of a prodomain, catalytic domain, hinge region, and a hemopexin domain. It is secreted as an inactive zymogen that becomes activated extracellularly. The most relevant natural activators of proMMP9 are unknown. MMP9 activation may be mediated by removal of the prodomain by serine proteases or other MMPs, or it may be a direct response to oxidative stress that disrupts the cysteine switch. MMP9 is capable of processing cytokines and chemokines. For example, it is reported to release the biologically active form of vascular endothelial growth factor (VEGF), which indicates that MMP9 may play a crucial role in the formation of new blood vessels. Increased MMP9 expression and activity has been shown to be related to various cancers, as it may contribute to the release of metastatic cells from the bulk tumor as well as their entrance at the site of metastasis by degrading proteins in the ECM and basement membrane.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant Human MMP-9
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration of 0.25µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human MMP-9 protein ranging from 5 to 0.078 ng/ml are recommended for each ELISA plate when paired with M2101G05 (Cat. No. 681802). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated M2108F07 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M2101G05 (Cat. No. 681802) antibody as the capture antibody.

Note: For testing human MMP-9 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 444907) is specially developed and recommended.

AB_2734495 (BioLegend Cat. No. 532101)

Antigen Details


MMP-9 is expressed by alveolar macrophages, granulocytes, fibroblasts, and fibrosarcoma cells.

Degradation of extracellular matrix (ECM) and connective tissue proteins, angiogenesis, tissue remodeling, and cancer metastasis. Regulates chemokine activity. Inhibited by TIMPs and α2-macroglobulin (inhibitor of MMPs in bodily fluids).
ECM proteins
Cell Type
Macrophages, Granulocytes, Fibroblasts
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Chakrabarti S, Patel KD. 2005. Exp. Lung Res. 31:599.
  2. Klein T, Bischoff R. 2011. Amino Acids. 41:271.
  3. Raffetto JD, Khalil RA. 2008. Biochem. Pharmacol. 75:346.
  4. Bourboulia D, Stetler-Stevenson WG. 2010. Semin. Cancer Biol. 20:161.
  5. Vilen ST, et al. 2013. Scientific World Journal. 920595.
Gene ID
4318 View all products for this Gene ID
View information about MMP-9 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 05/29/2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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  • Biotin anti-human MMP-9


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