APC/Cyanine7 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
405208 100 µg $193
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Cyanine7 is useful for detecting biotinylated antibodies. The excitation of APC/Cyanine7 by 600-635 nm laser light induces a fluorescence emission maximum of 780 nm.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin APC/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin APC/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Cyanine7 conjugation. For immunofluorescent staining, the recommended use of this reagent is =0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-Allophycocyanin/Cyanine7 (APC/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Cyanine7 is 350 kD and Streptavidin alone is 52 kD.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Maresz K, et al. 2005. J. Neurochemistry 95:437.
  2. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.PubMed.
  3. Ponomarev ED, et al. 2007. J. Immunol. 178:39.
  4. Mann MK, et al. 2007. J. Immunol. 178:3447.PubMed
  5. Ponomarev ED,et al.2005.J. Immunol Methods.300:32. PubMed
  6. Cao YA, et al. 2008. Blood PubMed
  7. Garidou L, et al. 2009. J. Virol. PubMed
  8. Dolgachev V, et al. 2009. J. Immunol. 183:5705. PubMed
  9. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed.
  10. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed
  11. Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed
  12. Prince J, et al. 2013. J. Immunol. 191:4709. PubMed
  13. Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed
  14. Orre M, et al. 2014. Neurbiol Aging. 35:1. PubMed
  15. Barbera M, et al. 2014. Gut. 64:11. PubMed
  16. Zhang Y, et al. 2014. Infect Immun. 82:1698. PubMed
  17. Peterson BC, et al. 2014. J Leukoc Biol. 95:809. PubMed
  18. Barbera M, et al. 2015. Gut. 64:11. PubMed
  19. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
Product Citations
  1. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  2. Izumi T, et al. 2013. Cytotherapy. 15:481. PubMed
  3. Hamanaka S, et al. 2013. Biochem Biophys Res Commun. 435:586. PubMed
  4. Prince J, et al. 2013. J Immunol. 191:4709. PubMed
  5. Phanse Y, et al. 2013. Acta Biomater. 9:8902. PubMed
  6. Orre M, et al. 2014. Neurobiol Aging. 35:1. PubMed
  7. Zhang Y, et al. 2014. Infect Immun . 82:1689. PubMed
  8. Petersen B, et al. 2014. J Leukoc Biol. 95:809. PubMed
  9. Barbera M, et al. 2015. Gut. 64:11. PubMed
  10. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  11. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  12. Seri Y, et al. 2015. Sci Rep. 5: 13041. PubMed
  13. Chao D, et al. 2015. PLoS One. 10: 0138107. PubMed
  14. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  15. Gillespie A, et al. 2016. PLoS One. 12: 1005419. PubMed
  16. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  17. Furusawa J, et al. 2016. PLoS Pathog. 12: 1005507. PubMed
  18. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
  19. Shimizu T, et al. 2016. J Exp Med. 213: 1479 - 1496. PubMed
  20. Lin K, et al. 2016. Sci Rep. 6:31444. PubMed
  21. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  22. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  23. Ritter M, et al. 2020. Ann Hematol. 99:2329. PubMed
  24. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  25. Kulkarni N, et al. 2017. Eur J Appl Physiol.. 10.1007/s00421-017-3704-z. PubMed
  26. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  27. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  28. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  29. Greenwald AC, et al. 2019. J Exp Med. 216:215. PubMed
  30. Gudjonsson A, et al. 2019. Front Immunol. 10:1086. PubMed
  31. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  32. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  33. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  34. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  35. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  36. Ponomarev ED, et al. 2005. J Immunol Methods. 300:32:00. PubMed
  37. Ponomarev E, et al. 2006. J Immunol. 176:1402. PubMed
  38. Mann M, et al. 2007. J Immunol . 178:3447. PubMed
  39. Cao Y, et al. 2008. Blood. 112:4494. PubMed
  40. Dolgachev V, et al. 2009. J Immunol. 183:5705. PubMed
  41. Garidou L, et al. 2009. J Virol. 83:8905. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login/Register
Remember me
Forgot your password? Reset Password
Request an Account