APC/Cyanine7 anti-human CD32B/C Antibody

Pricing & Availability
Clone
S18005H (See other available formats)
Other Names
FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32B, CD32C, FCG2, FCGR2, IGFR2, FcRII-c, CDW32
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
S18005H_APCCyanine7_CD32B_Antibody_121720
Human peripheral blood lymphocytes were stained with CD19 FITC and CD32B/C (clone S18005H) APC/Cyanine7 (left) or mouse IgG1, κ APC/Cyanine7 isotype control (right).
  • S18005H_APCCyanine7_CD32B_Antibody_121720
    Human peripheral blood lymphocytes were stained with CD19 FITC and CD32B/C (clone S18005H) APC/Cyanine7 (left) or mouse IgG1, κ APC/Cyanine7 isotype control (right).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
398311 25 tests $165
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Description

CD32B (FCGR2B) and CD32C (FCGR2C) are 40 kDa, type I transmembrane proteins that are members of the Ig superfamily of low-affinity immunoglobulin gamma Fc receptors. CD32B has a cytoplasmic tail that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), while CD32C contains an immunoreceptor tyrosine-based activation motif (ITAM). CD32B and CD32C are low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B and CD32C are expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.

RRID
AB_2890843 (BioLegend Cat. No. 398311)

Antigen Details

Structure
Type I transmembrane protein, 40 kDa, member of the Ig superfamily
Distribution

B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.

Function
Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation
Ligand/Receptor
Fc region of complex or aggregated immunoglobulins gamma
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Bruhns P, et al. 2009. Blood. 113: 3716.
  2. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735.
  3. Descours B, et al. 2017. Nature. 543: 564.
  4. Tomiyama Y, et al. 1992. Blood. 80: 2261.
  5. Indik Z, et al. 1991. J Clin Invest. 88: 1766.
  6. Ramsland PA, et al. 2011. J Immunol. 187: 3208.
  7. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311.
  8. Bournazos S, et al. 2009. J Immunol. 182: 8026.
  9. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437.
  10. Sandilands GP, et al. 1997. Immunology. 91: 204.
  11. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878.
  12. Gillis C, et al. 2014. Front Immunol. 5: 254.
Gene ID
2213 View all products for this Gene ID 9103 View all products for this Gene ID
UniProt
View information about CD32B CD32C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/17/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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