- 6B8B69 (See other available formats)
- Other Names
- P44/42 MAP (mitogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
- Mouse IgG2a, κ
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- Product Citations
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ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.
AB_2728374 (BioLegend Cat. No. 369521)
AB_2728375 (BioLegend Cat. No. 369522)
- ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Cytoplasm and the nucleus.
- ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
- ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
- Cell Type
- Biology Area
- Molecular Family
- Phospho-Proteins, Postsynaptic proteins, Protein Kinases/Phosphatase
- Antigen References
1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.
- Gene ID
- 5594 View all products for this Gene ID
- View information about ERK1/2 Phospho (Thr202/Tyr204) on UniProt.org
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