Anti-E. coli RNA Polymerase β Antibody

Pricing & Availability
Clone
8RB13 (See other available formats)
Other Names
RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Previously
NeoClone Catalog# W0023
Isotype
Mouse IgG1
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Product Citations
publications
1-8RB13_E.coliRNAPolymeraseBetaPrime_Antibody_WB_012419
Total cell lysates (15 µg total protein) from E. Coli BL21 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.01 µg/mL (1:100,000 dilution) of Purified anti-E. Coli RNA Polymerase β Antibody, clone 8RB13, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • 1-8RB13_E.coliRNAPolymeraseBetaPrime_Antibody_WB_012419
    Total cell lysates (15 µg total protein) from E. Coli BL21 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.01 µg/mL (1:100,000 dilution) of Purified anti-E. Coli RNA Polymerase β Antibody, clone 8RB13, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Lane M: Molecular Weight marker.
  • 2-8RB13_E.coliRNAPolymeraseBetaPrime_Antibody_WB_092116
    Total cell lysates (15 µg protein) from E. Coli strain BL21 and HeLa were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with Anti-E. Coli RNA Polymerase β Antibody (clone 8RB13) followed by HRP goat anti-mouse IgG antibody and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
663903 100 µL $275
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Description

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

Product Details
Technical data sheet

Product Details

Reactivity
E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant E. coli RNA polymerase β subunit.
Preparation
Dried ascites
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store lyophilized antibody at -20°C. Avoid repeat freeze/thaw cycles.
Application

WB - Quality tested
ELISA - Validated
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconsitution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.

Application References

(PubMed link indicates BioLegend citation)
  1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
  2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
  3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
Product Citations
  1. Broder U, Jaeger T, Jenal U 2016. Nat Microbiol. 2:16184. PubMed
  2. Heinrich K, et al. 2019. MBio. 10. PubMed
  3. Pribis JP et al. 2019. Mol Cell. 74(4):785-800 . PubMed
  4. Ju X, et al. 2019. Nat Microbiol. 1.490972222. PubMed
RRID
AB_2564524 (BioLegend Cat. No. 663903)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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