Alexa Fluor® 647 anti-mouse H-2Kq Antibody

Pricing & Availability
KH114 (See other available formats)
Other Names
MHC class I
Mouse (BALB/c) IgG2a, κ
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Product Citations
FVB mouse splenocytes stained with purified KH114 Alexa Fluor® 647
  • KH114_Alx647_020708
    FVB mouse splenocytes stained with purified KH114 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
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115106 100 µg $195
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The KH114 antibody reacts with the H-2Kq MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kq haplotype. H-2Kq is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The KH114 antibody has not been reported to cross-react with other haplotypes.

Product Details
Technical data sheet

Product Details

Mouse q haplotype
Antibody Type
Host Species
B10.Q mouse splenocytes
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1. Does not cross-react with other haplotypes (e.g. a, b, d, f, k, n, p, r, s, u, v).

Application References

(PubMed link indicates BioLegend citation)
  1. Hasendrug K, et al. 1987. Immunogenetics 25:136.
  2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
  3. Nanni P, et al. 2001. J. Exp. Med. 194:1195.
  4. Pastor WA, et al. 2014. Nat Commun. 12:5795. PubMed
Product Citations
  1. Pastor W, et al. 2014. Nat Commun. 12:5795. PubMed
  2. Collinson-Pautz M, et al. 2016. PLoS One. 11:e0164547. PubMed
Publication Library
AB_893562 (BioLegend Cat. No. 115106)

Antigen Details

MHC class I
Nucleated cells of the H-2Kq haplotype
Antigen presentation
Ligand Receptor
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
4. Hasendrug K, et al. 1987. Immunogenetics 25:136.

Gene ID
14972 View all products for this Gene ID
View information about H-2Kq on

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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