Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody

Pricing & Availability
Clone
4B11B69 (See other available formats)
Other Names
P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
4B11B69_A647_ERK1slash2_Phospho_Antibody_1_021216
Human peripheral blood lymphocytes were stimulated with (filled histogram), or without (open histogram), Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647.
  • 4B11B69_A647_ERK1slash2_Phospho_Antibody_1_021216
    Human peripheral blood lymphocytes were stimulated with (filled histogram), or without (open histogram), Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647.
  • 4B11B69_A647_ERK1slash2_Phospho_Antibody_2_101116
    C57BL/6 mouse splenocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
675503 25 tests $125
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675504 100 tests $295
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Description

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
RRID
AB_2571893 (BioLegend Cat. No. 675503)
AB_2571894 (BioLegend Cat. No. 675504)

Antigen Details

Structure
ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution

Cytoplasm. Nucleus.

Function
ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
Interaction
ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Phospho-Proteins, Postsynaptic proteins, Protein Kinases/Phosphatase
Antigen References

1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.

Gene ID
5594 View all products for this Gene ID
UniProt
View information about ERK1/2 Phospho on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 10/11/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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