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Alexa Fluor® 488 anti-RBFOX3 (NeuN) Antibody

Pricing & Availability
Clone
W18230A (See other available formats)
Regulatory Status
RUO
Other Names
NeuN, RNA binding protein fox-1 homolog 3 (RBFox3), hexaribonucleotide binding protein 3, HRNBP3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W18230A_AF-488_RBFOX3_IHC-F_040924
IHC staining of Alexa Fluor® 488 anti-RBFOX3 (NeuN) (clone W18230A) on frozen mouse brain tissue. The tissue was incubated without (panel A) and with (panel B) 5 µg/mL of Alexa Fluor® 488 anti- RBFOX3 (NeuN) (clone W18230A) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • a. W18230A_AF-488_RBFOX3_IHC-F_040924
    IHC staining of Alexa Fluor® 488 anti-RBFOX3 (NeuN) (clone W18230A) on frozen mouse brain tissue. The tissue was incubated without (panel A) and with (panel B) 5 µg/mL of Alexa Fluor® 488 anti- RBFOX3 (NeuN) (clone W18230A) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
  • b. W18230A_AF-488_RBFOX3_IHC-P_040924
    IHC staining of Alexa Fluor® 488 anti-RBFOX3 (NeuN) (clone W18230A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panels A and B) and with (panels C and D) 5 µg/mL of Alexa Fluor® 488 anti- RBFOX3 (NeuN) (clone W18230A) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801) (panels A and C). Images were captured with a 40X objective and merged (panels A and C). Scale bar: 50 µm
See Alexa Fluor® 488 spectral data
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608455 25 µg $190
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608456 100 µg $480
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Description

RBFox3, commonly referred to as NeuN, is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name RBFox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. RBFox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The antibody reveals strong nuclear and distal cytoplasmic staining for RBFox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This RBFox3/NeuN antibody is therefore an excellent marker of neuronal cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant human RBFOX3, isoform 5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested.  For immunohistochemistry in paraffin-embedded tissues, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

For immunohistochemistry in paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) results in dim signal. 

Antigen Details

Structure
RBFox3 is a 312 amino acid protein with a predicted molecular weight of 34 kD
Distribution

Mature Neurons, Neural Stem Cells/ Cytoplasm and Nucleus

Function
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Antigen References
  1. Dhekne HS, et al. 2018. Elife. 7:e40202.
  2. Sun Y, et al. 2020. J Neuroinflammation. 17:72. 
  3. Schwabenland M, et al. 2021. Immunity. 54:1594-1610. 
Gene ID
146713 View all products for this Gene ID
UniProt
View information about RBFOX3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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