Alexa Fluor® 488 anti-Cytokeratin (pan reactive) Antibody

Pricing & Availability
Clone
C-11 (See other available formats)
Other Names
Cytokeratin, Keratin
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cdash11_A488_Cytokeratin_PanReactive_Antibody_1_IF_062316
MCF-7 mammary adenocarcinoma cells were fixed, permeabilized and blocked according to standard protocols. The cells were stained with anti-Cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 488 (green) antibody. Nuclei were counterstained with DAPI (blue).
  • Cdash11_A488_Cytokeratin_PanReactive_Antibody_1_IF_062316
    MCF-7 mammary adenocarcinoma cells were fixed, permeabilized and blocked according to standard protocols. The cells were stained with anti-Cytokeratin (pan reactive) (clone C-11) Alexa Fluor® 488 (green) antibody. Nuclei were counterstained with DAPI (blue).
  • Cdash11_A488_Cytokeratin_PanReactive_Antibody_2_IHC_120216
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination, rehydration, antigen retrieval, and blocking. Tissue was stained with anti-Pan Cytokeratin (clone C-11) Alexa Fluor® 488 (green) antibody and anti-mouse/human CD44 (clone IM7) Alexa Fluor® 594 (red) antibody. Nuclei were counterstained with DAPI (blue). Expanded protocol available by following the Additional Data link.
See Alexa Fluor® 488 spectral data See expanded IF/IHC-P data...
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628608 100 µg $295
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Description

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.

Product Details
Technical data sheet

Product Details

Reactivity
Recognizes human cytokeratin 4, 5, 6, 8, 10, 13, and 18.
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Keratin enriched fraction from human epidermoid carcinoma.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1.25 - 5.0 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.

Application References

(PubMed link indicates BioLegend citation)
  1. Kovarik J. 1988. Int. J. Cancer Suppl. 3:50.
  2. Bartek J, et al. 1991. J. Pathol. 164:215.
  3. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed
  4. Comito G, et al. 2012. Cancer Lett. 324:31. PubMed
  5. Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow)
  6. Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC)
RRID
AB_2616664 (BioLegend Cat. No. 628608)

Antigen Details

Structure
Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution

Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus

Function
Intermediate filament protein involved with the cytoskeleton.
Interaction
Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
UniProt
View information about Cytokeratin (pan reactive) on UniProt.org

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Go To Top Version: 3    Revision Date: 12/02/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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