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HRP anti-Tau Phospho (Thr181) Antibody

Pricing & Availability
Clone
M7004D06 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule associated protein tau
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_M7004D06_HRP_Tau-Phospho_Antibody_2_041118
IHC staining of HRP anti-Tau Phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer’s disease (right panel) human brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
  • 1_M7004D06_HRP_Tau-Phospho_Antibody_2_041118
    IHC staining of HRP anti-Tau Phospho (Thr181) antibody (clone M7004D06) on formalin-fixed paraffin-embedded normal (left panel) and Alzheimer’s disease (right panel) human brain tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissues were incubated with 5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
  • 2_M7004D06_HRP_Tau-Phospho_Antibody_1_041118
    Western blot of HRP anti-Tau Phospho (Thr181) antibody (clone M7004D06). Lane 1: Molecular weight marker; Lane 2: 20 µg of Alzheimer’s disease human brain lysate; Lane 3: 20 µg of normal human brain lysate. The blot was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Recent studies indicate that cerebrospinal fluid tau phosphorylated at position threonine 181 has diagnostic utility for several neurological disorders. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain. Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
15-mer pT181 Tau peptide conjugated to KLH.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, Alpha-synuclein
Molecular Family
Phospho-Proteins, Tau
Antigen References

1. Meredith JE Jr, et al. 2013. PLoS One 8(10):e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1):89. PubMed

Gene ID
4137 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Tau Phospho Thr181
Specificity Alt (DOES NOT SHOW ON TDS):
Tau Phospho (Thr181)
App Abbreviation (DOES NOT SHOW ON TDS):
IHC-P,WB
UniProt
View information about Tau Phospho Thr181 on UniProt.org

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Go To Top Version: 0    Revision Date: 04/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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