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Ultra-LEAF™ Purified anti-human CD275 (B7-H2, ICOSL) Antibody

Pricing & Availability
Clone
W21036I (See other available formats)
Regulatory Status
RUO
Other Names
B7 homolog 2 (B7H2), GL50, B7 related protein 1 (B7RP1), CD275, inducible T cell costimulator ligand ICOS Ligand), LICOS, ICOSLG
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W21036I_ULEAF_CD275_Block_082823
Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera (Cat. No. 798704) (black circles) binds to immobilized human ICOS-Fc chimera (Cat. No. 599004). Ultra-LEAF™ purified anti-human CD275 (B7-H2, ICOSL) (clone W21036I) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.01 - 0.07 µg/mL.
  • W21036I_ULEAF_CD275_Block_082823
    Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera (Cat. No. 798704) (black circles) binds to immobilized human ICOS-Fc chimera (Cat. No. 599004). Ultra-LEAF™ purified anti-human CD275 (B7-H2, ICOSL) (clone W21036I) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ isotype control (Cat. No. 400544) (gray triangles) does not have this effect. ND50 range: 0.01 - 0.07 µg/mL.
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387505 100 µg 336€
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387506 1 mg 976€
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Description

CD275 (B7-H2, ICOSL) belongs to the B7 family of immunoregulatory molecules and is a transmembrane glycoprotein of approximately 60 kD. Mature human CD275 consists of 238 amino acid extracellular domain (ECD), two immunoglobulin-like domains, a 21 amino acid transmembrane segment, and a 25-residue cytoplasmic domain. Within the ECD, human CD275 shares 50% and 54% amino acid sequence homology with mouse and rat CD275, respectively. CD275 is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and in monocytes. It binds to ICOS and is expressed on activated T cells, which leads to both positive and negative effects on immune responses including its own downregulation. Mouse and human CD275 exhibit cross species binding to ICOS. CD275 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells, and germinal centers. In addition, human CD275 binds to CD28 and CTLA4. The interaction of human CD275 and CD28 can co-stimulate both human mouse naïve T cells and regulatory T cells (Treg). CD275 plays a role in the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lungs. CD275 activation by ICOS on Tregs limits pulmonary inflammation and airway hyper-responsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human B7-H2-Fc Chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.04 μg/mL Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera to 1 μg/mL immobilized human ICOS-Fc chimera. ND50 range: 0.01-0.07 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W21036I completely blocks clone 9F.8A4 but does not block clone 2D3.

RRID
AB_3083390 (BioLegend Cat. No. 387505)
AB_3083390 (BioLegend Cat. No. 387506)

Antigen Details

Structure
Homodimer
Distribution

B cells, macrophages, monocytes, and dendritic cells

Function
The CD275/ICOS signal induces T cell proliferation and secretion of both Th1 and Th2 cytokines, such as IL-4 and IFN-γ, but not IL-2 (it also induces IL-5 and TNF-α production). CD275/ICOS signal may favor the induction of Th2-type responses and thus may be involved in the negative regulation of cell-mediated immune responses against tumors. CD275 is induced by TNF-α in B cells and monocytes and downregulated in dendritic cells.
Interaction
Activated T cells
Ligand/Receptor
ICOS
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Ishikawa K, et al. 1998. DNA Res. 5:169-76.
  2. Yoshinaga SK, et al. 2000. Int. Immunol. 12:1439-47.
  3. Flesch IE. 2003. J. Biol. Regul. Homeost. Agents. 16:217-9.
  4. Akbari O, et al. 2002. Nat. Med. 8:1024-32.
  5. Yao S, et al. 2011. Immunity. 34:729-40.
Gene ID
23308 View all products for this Gene ID
UniProt
View information about CD275 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 08/28/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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