- Clone
- UCHL1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV N31
- Other Names
- CD45RO
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human PBMCs stained with 153Eu-anti-CD45RA (HI100) and 164Dy-anti-CD45RO (UCHL1). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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304239 | 100 µg | 104€ |
CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- IL-2 dependent T cell line, CA1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
CyTOF®, PG - Verified - Recommended Usage
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This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
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The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
- Additional Product Notes
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Maxpar® is a registered trademark of Standard BioTools Inc.
- Application References
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- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
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- RRID
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AB_2563752 (BioLegend Cat. No. 304239)
Antigen Details
- Structure
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
- Distribution
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Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Function
- Enhances TCR and BCR signaling
- Ligand/Receptor
- CD22
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85. - Gene ID
- 5788 View all products for this Gene ID
- UniProt
- View information about CD45RO on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
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We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
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We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
-
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
-
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other Formats
View All CD45RO Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD45RO
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FITC anti-human CD45RO
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PE anti-human CD45RO
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PE/Cyanine5 anti-human CD45RO
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Purified anti-human CD45RO
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Alexa Fluor® 488 anti-human CD45RO
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Pacific Blue™ anti-human CD45RO
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Alexa Fluor® 700 anti-human CD45RO
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Biotin anti-human CD45RO
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Brilliant Violet 421™ anti-human CD45RO
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PerCP/Cyanine5.5 anti-human CD45RO
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Brilliant Violet 570™ anti-human CD45RO
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Brilliant Violet 605™ anti-human CD45RO
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APC/Cyanine7 anti-human CD45RO
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PE/Cyanine7 anti-human CD45RO
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Brilliant Violet 650™ anti-human CD45RO
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Brilliant Violet 711™ anti-human CD45RO
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Brilliant Violet 785™ anti-human CD45RO
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Alexa Fluor® 594 anti-human CD45RO
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Purified anti-human CD45RO (Maxpar® Ready)
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Brilliant Violet 510™ anti-human CD45RO
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PE/Dazzle™ 594 anti-human CD45RO
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APC/Fire™ 750 anti-human CD45RO
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PerCP anti-human CD45RO
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APC anti-human CD45RO
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TotalSeq™-A0087 anti-human CD45RO
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TotalSeq™-B0087 anti-human CD45RO
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TotalSeq™-C0087 anti-human CD45RO
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Brilliant Violet 750™ anti-human CD45RO
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PE/Fire™ 640 anti-human CD45RO
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TotalSeq™-D0087 anti-human CD45RO
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Spark Violet™ 423 anti-human CD45RO
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GMP APC anti-human CD45RO
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PE anti-human CD45RO
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