Purified anti-human CD45RO Maxpar Ready Antibody anti-CD45RO

Pricing & Availability

Clone: UCHL1 (See other available formats)
Regulatory Status: RUO
Workshop: IV N31
Other Names: CD45RO
Isotype: Mouse IgG2a, κ
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Product Citations: publications

Human PBMCs stained with 153Eu-anti-CD45RA (HI100) and 164Dy-anti-CD45RO (UCHL1). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.

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304239

100 µg

104€

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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee, Cynomolgus, Common Marmoset

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

IL-2 dependent T cell line, CA1

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF®, PG - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁵ and formalin-fixed paraffin-embedded tissue sections⁴, Western blotting², and immunoprecipitation³.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
Yamada M, et al. 1996. Stroke 27:1155. (IHC)
Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

Product Citations

Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
Neidleman J, et al. 2020. Elife. 9:00. PubMed
Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
Neidleman J, et al. 2021. Elife. 10:. PubMed
Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed
Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed
Wimmers F, et al. 2021. Cell. 184:3915. PubMed
Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed

RRID

AB_2563752 (BioLegend Cat. No. 304239)

Antigen Details

Structure: Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution: Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
Function: Enhances TCR and BCR signaling
Ligand/Receptor: CD22
Cell Type: B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788 View all products for this Gene ID
UniProt: View information about CD45RO on UniProt.org

Documentation

Reviews

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD45RO Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD45RO	UCHL1	FC
FITC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC
PE/Cyanine5 anti-human CD45RO	UCHL1	FC
Purified anti-human CD45RO	UCHL1	FC,WB,IP,IHC-P
Alexa Fluor® 488 anti-human CD45RO	UCHL1	FC,IHC-P
Pacific Blue™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 700 anti-human CD45RO	UCHL1	FC
Biotin anti-human CD45RO	UCHL1	FC
Brilliant Violet 421™ anti-human CD45RO	UCHL1	FC,IHC-P
PerCP/Cyanine5.5 anti-human CD45RO	UCHL1	FC
Brilliant Violet 570™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 605™ anti-human CD45RO	UCHL1	FC
APC/Cyanine7 anti-human CD45RO	UCHL1	FC
PE/Cyanine7 anti-human CD45RO	UCHL1	FC
Brilliant Violet 650™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 711™ anti-human CD45RO	UCHL1	FC
Brilliant Violet 785™ anti-human CD45RO	UCHL1	FC
Alexa Fluor® 594 anti-human CD45RO	UCHL1	IHC-P
Purified anti-human CD45RO (Maxpar® Ready)	UCHL1	FC,CyTOF®
Brilliant Violet 510™ anti-human CD45RO	UCHL1	FC
PE/Dazzle™ 594 anti-human CD45RO	UCHL1	FC
APC/Fire™ 750 anti-human CD45RO	UCHL1	FC
PerCP anti-human CD45RO	UCHL1	FC
APC anti-human CD45RO	UCHL1	FC
TotalSeq™-A0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-B0087 anti-human CD45RO	UCHL1	PG
TotalSeq™-C0087 anti-human CD45RO	UCHL1	PG
Brilliant Violet 750™ anti-human CD45RO	UCHL1	FC
PE/Fire™ 640 anti-human CD45RO	UCHL1	FC
TotalSeq™-D0087 anti-human CD45RO	UCHL1	PG
Spark Violet™ 423 anti-human CD45RO	UCHL1	FC
GMP APC anti-human CD45RO	UCHL1	FC
PE anti-human CD45RO	UCHL1	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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