PerCP/Cyanine5.5 anti-human CD49b Antibody

Pricing & Availability
P1E6-C5 (See other available formats)
Regulatory Status
Other Names
α2 integrin, VLA-2 α chain, Integrin α2 chain
Mouse IgG1, κ
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Product Citations
Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • P1E6-C5_PerCPCyanine55_CD49b_Antibody_101419
    Human peripheral blood platelets were stained with CD49b (clone P1E6-C5) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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359315 25 tests 132€
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359316 100 tests 287€
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CD49b is a 170 kD transmembrane protein, also known as α2 integrin, VLA-2 α chain, Integrin α2 and GPIa. It associates with CD29 (β1 integrin) to form VLA-2, a collagen and laminin receptor on many cell types including monocytes, platelets, activated T cells, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts. CD49b has been reported to interact with F-actin and matrix metalloproteinase 1. CD49b is a platelet alloantigen and has been associated with neonatal alloimmune thrombocytopenia. Deficiencies in this protein have been associated with hemorrhagic disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
HT1080 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vitro blocking activity1,2, immunoprecipitation3, and ELISA4

Application References
  1. Hirsch MS, et al. 1997. Dev. Dyn. 210:249. (Block)
  2. Sawhney RS, et al. 2006. J. Biol. Chem. 281:8497. (Block)
  3. Lee SA, et al. 2009. Carcinogensis. 30:1872. (IP)
  4. Zßrate S, et al. 2004. J. Virol. 78:10839. (ELISA)
AB_2820014 (BioLegend Cat. No. 359315)
AB_2820015 (BioLegend Cat. No. 359316)

Antigen Details

Integrin alpha chain family, type I membrane protein, alpha chain of heterodimeric integrin receptor, 170 kD; associates with CD29 to form collagen, laminin receptor

Broadly expressed on many cell types including B cells, monocytes, platelets, megakaryocytes, neuronal cells, epithelial cells, and osteoclasts

With CD29; binds collagen and laminin
Forms heterodimer with integrin β1 (CD29) which is a receptor for laminin and collagen
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kaplan C, et al. 1991. Br. J. Haematol. 78:425.
2. Kiefel V, et al. 1991. Vox Sang. 60:244.
3. Nieuwenhuis HK, et al. 1985. Nature 318:470.
4. Takada Y and Helmer ME. 1989. J. Cell Biol. 109:397.

Gene ID
3673 View all products for this Gene ID
View information about CD49b on

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10/14/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD49b

  • FITC anti-human CD49b

  • PE anti-human CD49b

  • APC anti-human CD49b

  • TotalSeq™-A0371 anti-human CD49b

  • PerCP/Cyanine5.5 anti-human CD49b

  • PE/Cyanine7 anti-human CD49b

  • TotalSeq™-C0371 anti-human CD49b

  • TotalSeq™-B0371 anti-human CD49b


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