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PE anti-human IL-21 Antibody

Pricing & Availability
Clone
3A3-N2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 21
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_3A3-N2_PE_091509
PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2
  • A_3A3-N2_PE_091509
    PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2
  • B_3A3-N2_PE_091509
Compare all formats See PE spectral data
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513003 25 tests 137€
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513004 100 tests 273€
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Description

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset, and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation, suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-21
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed
  2. Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
  3. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  4. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  5. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
RRID
AB_1877230 (BioLegend Cat. No. 513003)
AB_2249025 (BioLegend Cat. No. 513004)

Antigen Details

Distribution

Th17

Function
Immunomodulation
Cell Sources
NK-T cells and T cells
Cell Targets
Cytotoxic T cells (CTL) and Natural Killer (NK) cells that can destroy malignant or infected cells
Receptors
IL-21R
Cell Type
Th17
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nurieva R. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57.

Gene ID
59067 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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